Anti-inflammatory activity of aronia berry extracts in murine splenocytes

• Published in: Journal of functional foods Vol. 8; pp. 68 - 75
• Main Authors: Martin, Derek A., Taheri, Rod, Brand, Mark H., Draghi, Andrew, Sylvester, Francisco A., Bolling, Bradley W.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.05.2014; Elsevier
• Subjects: Anthocyanin; Anti-inflammatory; Aronia; Chokeberry; Immune; Polyphenol; Anthocyanin; Polyphenol; Chokeberry; Aronia; Anti-inflammatory; Immune
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2014.03.004

•Aronia ‘Viking’ extract increased IL-10 in mouse splenocytes.•Underutilized aronia extracts inhibited IL-6 in CD4+ and CD4− mouse splenocytes.•Minor polyphenols may be responsible for the anti-inflammatory activity of aronia. Aronia berries are a rich source of dietary polyphenols, with diverse polyphenol profiles among its genotypes. The objective of this work was to characterize the anti-inflammatory effects of underutilized aronia berries and their polyphenols using primary C57/BL6 mouse splenocytes. At 125 μg gallic acid equivalents/mL, the commercial ‘Viking’ aronia berry and underutilized aronia extracts inhibited LPS-stimulated IL-6 to a similar extent. ‘Viking’ extracts inhibited IL-6 predominately in CD4− lymphocytes. The primary polyphenol constituents of extracts were subsequently evaluated for inhibition of LPS-stimulated IL-6. Cyanidin-3-arabinoside, but not the primary aronia anthocyanin cyanidin-3-galactoside, inhibited IL-6 at 10 μg/mL. Quercetin, but not its 3-galactoside or glucoside, inhibited LPS-stimulated IL-6. Quercetin also inhibited LPS-stimulated IL-10, whereas ‘Viking’ extract increased splenocyte IL-10 in the absence of LPS. Thus, the capacity of aronia extracts to modulate LPS-stimulated splenocyte IL-6 and IL-10 in vitro was not attributed to its principal polyphenols.