The use of acid phosphatase test papers for DNA profiling

The acid phosphatase (AP) test is a routine assay used to screen casework items for the possible presence of semen. This colour test is carried out on filter paper which is retained after testing. Two-year-old AP test papers were found to contain sufficient DNA for short tandem repeat (STR) profilin...

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Bibliographic Details
Published inScience & justice Vol. 45; no. 2; pp. 97 - 102
Main Authors Reshef, A., Barash, M., Gallili, N., Michael, A., Brauner, P.
Format Journal Article
LanguageEnglish
Published England Elsevier Ireland Ltd 01.04.2005
Subjects
Acid Phosphatase - analysis
Acid phosphatase test papers
Adolescent
Clinical Enzyme Tests - methods
DNA Fingerprinting - methods
DNA profiling
Forensic Medicine - methods
Humans
Male
Paper - standards
post-conviction DNA testing
Semen - enzymology
SGM plus
short tandem repeats
silver staining
Tandem Repeat Sequences
Time Factors
SGM plus
silver staining
post-conviction DNA testing
Acid phosphatase test papers
short tandem repeats
DNA profiling
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Summary:The acid phosphatase (AP) test is a routine assay used to screen casework items for the possible presence of semen. This colour test is carried out on filter paper which is retained after testing. Two-year-old AP test papers were found to contain sufficient DNA for short tandem repeat (STR) profiling. Prior to polymerase chain reaction (PCR) amplification, the DNA was preferentially separated into sperm depleted and sperm enriched cell fractions. The implication of these findings for past and present cases is discussed.
Bibliography:ObjectType-Case Study-2
SourceType-Scholarly Journals-1
ObjectType-Feature-4
content type line 23
ObjectType-Report-1
ObjectType-Article-3
ISSN:1355-0306
1876-4452
DOI:10.1016/S1355-0306(05)71635-X