The Expression of Endothelin Receptor B in Melanoma Cells A375 and Sk-mel-1 and the Proliferative Effects of Endothelin 3 on A375 Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 27; no. 5; pp. 611 - 613
• Main Author: 林能兴 黄长征 田进 陶娟 张进 杨凌云 李延 刘业强 陈思远 沈关心 李家文 王椿森 余亚庭
• Format: Journal Article
• Language: English
• Published: China Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Chinap%Faculty of Immunology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 01.10.2007
• Subjects: A375细胞; Cell Line, Tumor; Cell Proliferation - drug effects; Endothelin-3 - pharmacology; Humans; Melanoma - metabolism; Melanoma - pathology; Receptor, Endothelin B - metabolism; Reverse Transcriptase Polymerase Chain Reaction; 内皮受体B; 细胞增殖; 黑素瘤; endothelin receptor B; melanoma; endothelin 3; SK-mel-1; A375
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0535-x

In order to investigate the expression of endothelin receptor B （ETR-B） in human malignant melanoma （MM） cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 （ET3） on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.