Co-assembly of HPV capsid proteins and aggregation-induced emission fluorogens for improved cell imaging

In order to improve the cell-imaging ability, and particularly, to extend the bio-application of AIEgen, human papillomavirus (HPV) capsid protein L1 was assembled with the complex of DNA and aggregation-induced emission fluorogen 9,10-distyrylhydrazine (DSAI), where the virus-like particles (VLPs)...

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Bibliographic Details
Published inNanoscale Vol. 12; no. 9; pp. 551 - 556
Main Authors Jing, Jiangbo, Xue, Ya-Rong, Liu, Yu-Xue, Xu, Bin, Li, Hong-Wei, Liu, Leijing, Wu, Yuqing, Tian, Wenjing
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 07.03.2020
Subjects
Agglomeration
Biocompatibility
Deoxyribonucleic acid
DNA
Emission
Fluorescence
Human papillomavirus
Imaging
Nanoparticles
Proteins
Viruses
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Summary:In order to improve the cell-imaging ability, and particularly, to extend the bio-application of AIEgen, human papillomavirus (HPV) capsid protein L1 was assembled with the complex of DNA and aggregation-induced emission fluorogen 9,10-distyrylhydrazine (DSAI), where the virus-like particles (VLPs) of HPV encapsulate the complex via electrostatic interaction. The co-assembled nanoparticles, DSAI-DNA@VLPs, showed homogeneous size (∼53 nm), enhanced fluorescence (8 × 2.5-fold), considerable stability (anti-DNase digestion), improved biocompatibility and commendable protection for the DSAI-DNA complex, ensuring virtual brighter imaging in live cells, both for HeLa and normal 293T cell lines. A co-assembly of HPV capsid protein L1 and an AIE molecule DSAI has been constructed via the bridge of dsDNA, ensuring their improved ability for cell imaging.
Bibliography:10.1039/c9nr09084c
Electronic supplementary information (ESI) available: Experimental details and supporting figures. See DOI
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ISSN:2040-3364
2040-3372
DOI:10.1039/c9nr09084c