The transcription factor FgStuA regulates virulence and mycotoxin biosynthesis via recruiting the SAGA complex in Fusarium graminearum

• Published in: The New phytologist Vol. 240; no. 6; pp. 2455 - 2467
• Main Authors: Xu, Chaoyun, Wang, Jing, Zhang, Yueqi, Luo, Yuming, Zhao, Youfu, Chen, Yun, Ma, Zhonghua
• Format: Journal Article
• Language: English
• Published: Lancaster Wiley Subscription Services, Inc 01.12.2023
• Subjects: Acetylation; Acetyltransferase; APSES‐type transcription factor; Biosynthesis; Deoxynivalenol; Fungi; Fusarium graminearum; Fusarium graminearum complex; Gene expression; Histones; Metabolism; multigene family; mycotoxin biosynthesis; Mycotoxins; Pathogens; Phytopathogenic fungi; Promoters; Regulatory mechanisms (biology); the Spt‐Ada‐Gcn5‐Acetyltransferase (SAGA) complex; Transcription; Transcription factors; Virulence
• ISSN: 0028-646X; 1469-8137; 1469-8137
• DOI: 10.1111/nph.19297

Summary The conserved Spt‐Ada‐Gcn5‐Acetyltransferase (SAGA) complex controls eukaryotic transcription by modifying acetylation of histones. However, the mechanisms for this complex in regulating the transcription of target‐specific genes remain largely unknown in phytopathogenic fungi. A filamentous fungal‐specific transcription factor FgStuA was identified to interact with the SAGA complex physically. The coordinative mechanisms of FgStuA with the SAGA complex in regulating secondary metabolism and virulence were investigated in Fusarium graminearum with genetic, biochemical and molecular techniques. The transcription factor FgStuA binds to a 7‐bp cis‐element (BVTGCAK) of its target gene promoter. Under mycotoxin deoxynivalenol (DON) induction conditions, FgStuA recruits the SAGA complex into the promoter of TRI6, a core regulator of the DON biosynthesis gene cluster, leading to enhanced transcription of TRI6. During this process, we found that FgStuA is subject to acetylation by the SAGA complex, and acetylation of FgStuA plays a critical role for its enrichment in the TRI6 promoter. In addition, FgStuA together with the SAGA complex modulates fungal virulence. This study uncovers a novel regulatory mechanism of a transcription factor, which recruits and interacts with the SAGA complex to activate specific gene expression in pathogenic fungi.