Reduction of Aflatoxin B1 in Stored Peanuts (Arachis hypogaea L.) Using Saccharomyces cerevisiae

• Published in: Journal of food protection Vol. 74; no. 6; pp. 1003 - 1006
• Main Authors: PRADO, G, CRUZ MADEIRA, J. E. G, MORAIS, V. A. D, OLIVEIRA, M. S, SOUZA, R. A, PELUZIO, J. M, GODOY, I. J, SILVA, J. F. M, PIMENTA, R. S
• Format: Journal Article
• Language: English
• Published: Des Moines, IA International Association for Food Protection 01.06.2011
• Subjects: Aflatoxin B1 - biosynthesis; Arachis - chemistry; Aspergillus - growth & development; Aspergillus - metabolism; Biological and medical sciences; Consumer Product Safety; Food industries; Food microbiology; Food toxicology; Fundamental and applied biological sciences. Psychology; Humans; Pest Control, Biological - methods; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Temperature; Time Factors; Ascomycota; Peanut; Yeast; Mycotoxin; Arachis hypogaea; Fungi; Toxin; Reduction; Leguminosae; Dicotyledones; Angiospermae; Aflatoxin B1; Spermatophyta; Saccharomyces cerevisiae
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028x.jfp-10-380

Aflatoxin B(1) is a toxigenic and carcinogenic compound produced by Aspergillus flavus and Aspergillus parasiticus. To inhibit aflatoxin contamination of peanuts, seeds of two peanut breeds, IAC Caiapó and IAC Runner 886, were inoculated with A. parasiticus (1.0 × 10(6) spores per ml) and the yeast Saccharomyces cerevisiae (3.2 × 10(7) cells per ml) and incubated at 25°C for 7 and 15 days. Two experiments were conducted for each incubation period separately. The treatments were completely randomized, with three replications per treatment. Treatments included the two cultivars and three types of inoculation (pathogen alone, yeast and pathogen, and yeast 3 h before pathogen). Aflatoxin B(1) was quantified with a densitometer at 366 nm after thin layer chromatography. Aflatoxin B(1) contamination in peanuts was reduced after the addition of S. cerevisiae. The concentration of aflatoxin B(1) decreased by 74.4 and 55.9% after 7 and 15 days, respectively. The greatest aflatoxin reduction was observed when S. cerevisiae was inoculated 3 h before the pathogen in IAC Caiapó seeds and incubated for 7 days at 25°C. The use of S. cerevisiae is a promising strategy for biological control of aflatoxin contamination in peanuts.