Xuebijing injection induces anti-inflammatory-like effects and downregulates the expression of TLR4 and NF-κB in lung injury caused by dichlorvos poisoning

• Published in: Biomedicine & pharmacotherapy Vol. 106; pp. 1404 - 1411
• Main Authors: He, Fei, Wang, Jun, Liu, Yao, Wang, Xiaojing, Cai, Nan, Wu, Chao, Gao, Qingling
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.10.2018
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2018.07.111

•XBJ injection diminishes the damage of lung histopathology and improves oxygenation with DDVP poisoning.•XBJ injection prevents DDVP poisoning induced lung injury by attenuating the local and systemic inflammatory response.•The protective effect of XBJ injection appears to be mediated through downregulation of the TLR4 and NF-κB expression. The mechanism in lung injury caused by acute organophosphate pesticide poisoning (AOPP) and an effective treatment remains unclear. We aim to clarify how the inflammatory lung injury caused by AOPP might be modulated by Xuebijing (XBJ) injection. AOPP-induced lung injury model was induced by dichlorvos (DDVP) subcutaneous administration in rats and XBJ injection was administered by intraperitoneal injection after DDVP challenge. The effects of XBJ injection were assessed by lung histopathological analysis and lung injury scores, lung wet-to-dry weight ratios (WDR) and oxygenation, differential immune cell count in bronchoalveolar lavage fluid (BALF), IL-6 and TNF-α levels in blood, the levels of TLR4 and NF-κB proteins in lung tissue and blood acetylcholinesterase (AChE) activity. DDVP administration resulted in damage of lung histopathology and lower PaO2/FiO2 ratios (P < 0.05), which were notably attenuated by XBJ injection (P < 0.05). Total cell, macrophage, and neutrophils count in BALF and TNF-α and IL-6 levels in blood were significantly increased after DDVP exposure (P < 0.05), which were notably ameliorated by XBJ injection (P < 0.05). TLR4 and NF-κB protein in lung tissue expression after DDVP challenge were markedly increased (P < 0.05), and they were substantially downregulated by XBJ injection (P < 0.05). In addition, blood AChE activity was significantly decreased by DDVP administration (P < 0.05), however, there was no significant improvement after XBJ injection. XBJ injection prevents DDVP poisoning induced lung injury by attenuating the inflammatory response. The protective effect appears to be mediated through downregulation of the TLR4 and NF-κB expression.