Protective effects of puerarin on liver tissue in Salmonella-infected chicks: a proteomic analysis

• Published in: Poultry science Vol. 103; no. 1; p. 103281
• Main Authors: Lu, Yu, Guo, Xiaotong, Xu, Fengge, Wang, Fengyi, Wu, Hongsong, Bai, Yongjiang, Li, Wenhua, Zhang, Guisheng, Yuan, Jinbao, Pang, Quanhai
• Format: Journal Article
• Language: English
• Published: England Elsevier 01.01.2024
• Subjects: Animals; Anti-Bacterial Agents - metabolism; Biomarkers - metabolism; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - microbiology; Carcinoma, Hepatocellular - pathology; Carcinoma, Hepatocellular - veterinary; chick; Chickens - metabolism; Chickens - microbiology; Food Safety; Isoflavones - administration & dosage; liver; Liver - drug effects; Liver - metabolism; Liver - microbiology; Liver Neoplasms - metabolism; Liver Neoplasms - microbiology; Liver Neoplasms - pathology; Liver Neoplasms - veterinary; Oxidative Stress; proteomic; Proteomics; puerarin; Salmonella - drug effects; Salmonella enterica; Salmonella Infections, Animal - complications; Salmonella Infections, Animal - drug therapy; Salmonella Infections, Animal - microbiology; Salmonella enterica; proteomic; puerarin; liver; chick
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2023.103281

Salmonella enterica is a zoonotic bacterium that not only causes serious economic losses to the livestock and poultry industries but also seriously endangers human health. Long-term indiscriminate use of antibiotics has led to drug resistance in Salmonella, and thus the identification of alternatives to antibiotics is crucial. In this study, the effects of puerarin on the S. enterica-infected chickens were investigated. A total of 360 chicks were randomly assigned as the control group (CON), the S. enterica group (S), and puerarin-treatment group (P). Chicks in the P group were fed the basal diet supplemented with 50 (P50), 100 (P100), 200 (P200), and 400 (P400) mg/kg puerarin, respectively. It was found that puerarin treatment markedly altered the serum activities of aspartate aminotransferase (AST), alanine transaminase (ALT), and superoxide dismutase (SOD), together with the malondialdehyde (MDA) and total antioxidant capacity (T-AOC) contents in the serum. The mRNA expression of IL-6, IL-1β, TNF-α, Bcl-2, and caspase-8 in the livers of S. enterica-infected chicks was increased after infection but significantly reduced after treatment with puerarin. Histologic analysis showed that puerarin effectively mitigated morphological damage in the liver caused by S. enterica. Proteomic analysis revealed that S. enterica infection led to metabolic disorders in the liver, resulting in oxidative stress, increased inflammation, and significantly elevated levels of hepatocellular carcinoma biomarkers. The findings of the filtered sequencing were verified by using quantitative PCR (qPCR). Treatment with 100 mg/mL puerarin thus effectively alleviated disordered liver metabolism, reduced inflammation and oxidative damage and significantly reduced the levels of hepatocellular carcinoma biomarkers in the liver. The results suggest that puerarin has the potential to replace antibiotics to control Salmonella infection in poultry and thus improve food safety.