Propofol effects on cerebellar long-term depression

• Published in: Neuroscience letters Vol. 609; pp. 18 - 22
• Main Authors: Lee, Kwan Young, Kim, Young Im, Kim, Se Hoon, Park, Hyung Seo, Park, Youn Joon, Ha, Myung Sook, Jin, Yunju, Kim, Dong Kwan
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 16.11.2015
• Subjects: Anesthetics, Intravenous - pharmacology; Animals; Cerebellum; Cerebellum - drug effects; Cerebellum - physiology; In Vitro Techniques; Long-term depression; Long-Term Synaptic Depression - drug effects; Metabotropic glutamate receptor 1; Parallel fiber; Propofol; Propofol - pharmacology; Purkinje cell; Purkinje Cells - drug effects; Purkinje Cells - physiology; Rats, Wistar; Receptors, Metabotropic Glutamate - metabolism; Cerebellum; Parallel fiber; AMPA; D-Ser; DMSO; GluD2; LTP; PKC; GABAA; PC; PF; TRPC; aCSF; Metabotropic glutamate receptor 1; mGlyRl; Purkinje cell; DHPG; Propofol; LTD; Long-term depression
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2015.09.037

•The effect of propofol was studied in cerebellar parallel fiber-Purkinje cell synapses.•Propofol impaired cerebellar long-term synaptic plastcitiy.•Propofol suppressed mGluR1 activity in cerebellar Purkinje cells. Propofol is an intravenously administered anesthetic that induces γ-aminobutyric acid-mediated inhibition in the central nervous system. It has been implicated in prolonged movement disorders. Since the cerebellum is important for motor coordination and learning, we investigated the potential effects of propofol on cerebellar circuitry. Using the whole-cell patch-clamp technique in Wister rat cerebellar slices, we demonstrated that propofol administration impaired long-term depression from the parallel fiber (PF) to Purkinje cell (PC) synapses (PF–LTD). Also, propofol reduced metabotropic glutamate receptor 1 (mGluR1)-mediated and group I mGluR agonist-induced slow currents in PCs. These results suggest that the propofol-induced PF–LTD impairment may be related to an alteration in mGluR1 signaling, which is essential to motor learning.