Role of Connective Tissue Growth Factor in Extracellular Matrix Degradation in Renal Tubular Epithelial Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 27; no. 1; pp. 44 - 47
• Main Author: 张春 朱忠华 刘建社 杨晓 付玲 邓安国
• Format: Journal Article
• Language: English
• Published: China Department of Nephrology, Union Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 01.02.2007
• Subjects: Cell Line; Connective Tissue Growth Factor; Epithelial Cells - drug effects; Extracellular Matrix - metabolism; Humans; Immediate-Early Proteins - genetics; Immediate-Early Proteins - pharmacology; Immediate-Early Proteins - physiology; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - pharmacology; Intercellular Signaling Peptides and Proteins - physiology; Kidney Tubules - drug effects; Oligonucleotides, Antisense - pharmacology; Plasminogen Activator Inhibitor 1 - genetics; Plasminogen Activator Inhibitor 1 - secretion; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; Time Factors; Transfection; Transforming Growth Factor beta - pharmacology; Transforming Growth Factor beta1 - pharmacology; 上皮细胞; 细胞外物质; 肾疾病; 连接组织生长因子; connective tissue growth factor; plasminogen activator inhibitor-1; renal tubular epithelial cells; antisense oligodeoxynucleotide
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0113-2

In order to investigate the effects of connective tissue growth factor （CTGF） antisense oligodeoxynucleotide （ODN） on plasminogen activator inhibitor-1 （PAI-1） expression in renal tubular cells induced by transforming growth factor β1 （TGF-β1） and to explore the role of CTGF in the degradation of renal extracellular matrix （ECM）, a human proximal tubular epithelial cell line （HKC） was cultured in vitro. Cationic lipid-mediated CTGF antisense ODN was transfected into HKC. After HKC were stimulated with TGF-β1 （5 μg/L）, the mRNA level of PAI-1 was detected by RT-PCR. Intracellular PAI-1 protein synthesis was assessed by flow cytometry. The secreted PAI-1 in the media was determined by Western blot. The results showed that TGF-β1 could induce tubular CTGF and PAI-1 mRNA expression. The PAI-1 mRNA expression induced by TGF-β1 was significantly inhibited by CTGF antisense ODN. CTGF antisense ODN also inhibited intracellular PAI-1 protein synthesis and lowered the levels of PAI-1 protein secreted into the media. It was concluded that CTGF might play a crucial role in the degradation of excessive ECM during tubulointerstitial fibrosis, and blocking the biological effect of CTGF may he a novel way in preventing renal fibrosis.