Long noncoding RNAs interact with mRNAs: A new perspective on the mechanism of premature brain injury

• Published in: Neuroscience letters Vol. 707; p. 134274
• Main Authors: Chen, Rujuan, Piao, Xianhua, Xiao, Mi, Wang, Fan, Liu, Li
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 10.08.2019
• Subjects: Animals; Animals, Newborn; Brain - drug effects; Brain - embryology; Brain - metabolism; Brain - pathology; Brain injury; Encephalitis - metabolism; Encephalitis - pathology; Female; Inflammation; Lipopolysaccharides - pharmacology; Long noncoding RNA; Male; Maternal Exposure; Mice, Inbred BALB C; Preterm birth; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal Transduction; Inflammation; Preterm birth; Long noncoding RNA; Brain injury
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2019.05.028

•This study successfully built a premature brain injury animal model.•This study described the expression profile of lncRNAs and mRNAs in premature mice by using microarray technology.•This study investigates differentially expressed lncRNAs and mRNAs as well as their interactions in premature brain.•This study revealed a novel mechanism of premature brain development from a new perspective of lncRNAs and mRNAs coexpression network, and providing important thoughts for the therapy of premature brain injury. The molecular mechanism of premature brain injury induced by inflammation is not fully understood. Long noncoding RNAs (lncRNAs) have been reported to play crucial roles in neurological disorders including brain injury. However, little is known about the regulatory function of lncRNAs in the premature brain. This study investigates differentially expressed lncRNAs and mRNAs as well as their interactions in the premature brain. Lipopolysaccharides (LPS) were used to induce inflammation in premature rodent models. Brain histology was observed via hematoxylin and eosin (HE) staining and CD68 immunostaining. Arraystar microarry was designed for the profiling of differentially expressed lncRNAs and mRNAs in 4 LPS induced premature brains (L group), 4 full-term control brains (C group) and 3 premature brains were not induced by LPS (P group). Bioinformatic analysis was applied to reveal the functions and co-expression relationship of lncRNAs and mRNAs. Three lncRNAs and 2 mRNAs were selected for validation applying quantitative real time polymerase chain reaction (qRT-PCR). This study demonstrates dysregulated lncRNA and mRNA profiles in the premature brains upon inflammatory insult, thus revealing a novel mechanism of premature brain development from a new perspective of the lncRNAs and mRNA coexpression network and providing important insights into the therapy of premature brain injury.