Phytochemical investigation and antifeedant activity of Premna latifolia leaves

• Published in: Natural product research Vol. 25; no. 18; pp. 1680 - 1686
• Main Authors: Kumar, Ashok, Lal Tamta, Mahendra, Negi, Nisha, Chandrasekhar, K., Singh Negi, Devendra
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.10.2011
• Subjects: Animals; antifeedant activity; Chromatography, Gas; essential oil constituents; fatty acid analysis; Fatty Acids - analysis; Gas Chromatography-Mass Spectrometry; Hexanes; India; Larva - drug effects; Larva - growth & development; Oils, Volatile - analysis; Oils, Volatile - pharmacology; Palmitic Acid - analysis; Palmitic Acid - isolation & purification; Plant Leaves - chemistry; Premna latifolia; Spodoptera - drug effects; Spodoptera - growth & development; Spodoptera litura; Stearic Acids - analysis; Stearic Acids - isolation & purification; Verbenaceae - chemistry; India
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2010.511620

The essential oil of Premna latifolia Roxb. was obtained by hydrodistillation of fresh leaves of the plant having an oil yield of 0.05%, both non-polar and essential oil were analysed by GC and GC-MS. Hexane fraction of the leaves of P. latifolia was transesterified and analysed by GC and GC-MS, 40 non-polar components were identified comprising 89.3%. The most abundant fatty acid constituents were hexadecanoic acid (25.04%), 8,11,14-docosatrienoic acid (13.62%), octadecanoic acid (6.82%), 9,12-octadecadienoic acid (4.19%) and 29 components were investigated in the essential oil which comprises 78.1%. The most abundant oil constituents were 1-octen-3-ol (35.69%), terpendiol II (7.19%), δ-guaiene (7.49%) 2-undecanone (4.80%) and α-pinene (3.27%). Different extracts were also tested against polyphagous crop pest Spodoptera litura for antifeedant activity. Essential oil showed maximum growth reduction of 56.83% followed by chloroform extract of 43.93%.