Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development

Using the method of femtosecond laser surgery we study the fusion of two-cell mouse embryos under the action of tightly focused femtosecond laser radiation with the fusion efficiency reaching 60 %. The detailed statistical analysis of the efficiency of blastomere fusion and development of the embryo...

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Published inQuantum electronics (Woodbury, N.Y.) Vol. 45; no. 5; pp. 498 - 502
Main Authors Osychenko, A.A., Zalessky, A.D., Krivokharchenko, A.S., Shakhbazian, A.K., Ryabova, A.V., Nadtochenko, V.A.
Format Journal Article
LanguageEnglish
Published United States Turpion Ltd and the Russian Academy of Sciences 01.01.2015
Subjects
CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS
EFFICIENCY
EMBRYOS
Femtosecond
Femtosecond pulses
Formations
laser nanosurgery
LASER RADIATION
Lasers
MICE
preimplantation development
PULSES
Quantum electronics
Strain
STRAINS
SURGERY
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Summary:Using the method of femtosecond laser surgery we study the fusion of two-cell mouse embryos under the action of tightly focused femtosecond laser radiation with the fusion efficiency reaching 60 %. The detailed statistical analysis of the efficiency of blastomere fusion and development of the embryo up to the blastocyst stage after exposure of the embryos from different mice to a femtosecond pulse is presented. It is shown that the efficiency of blastocyst formation essentially depends on the biological characteristics of the embryo, namely, the strain and age of the donor mouse. The possibility of obtaining hexaploid embryonal cells using the methods of femtosecond laser surgery is demonstrated.
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ISSN:1063-7818
1468-4799
DOI:10.1070/QE2015v045n05ABEH015767