Nicotine modulates the facial stimulation-evoked responses in cerebellar granule cell layer in vivo in mice

• Published in: European journal of pharmacology Vol. 843; pp. 126 - 133
• Main Authors: Xu, Yin-Hua, Zhang, Bin-Bin, Su, Wen-Hao, Wu, Mao-Cheng, Bing, Yan-Hua, Cui, Song-Biao, Chu, Chun-Ping, Li, Yu-Zi, Qiu, De-Lai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2019
• Subjects: Animals; Cerebellar granule cell layer; Cerebellum - cytology; Cerebellum - physiology; Face; Female; Field potential recording; Male; Mice, Inbred ICR; Nicotine - pharmacology; Nicotinic acetylcholine receptors; Nicotinic Agonists - pharmacology; Physical Stimulation; Receptors, Nicotinic - physiology; Sensory stimulation; Synaptic Transmission; Sensory stimulation; Cerebellar granule cell layer; Nicotinic acetylcholine receptors; Field potential recording
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2018.11.022

Nicotinic acetylcholine receptors are cationic channels that mediate fast excitatory transmission in the central nervous system. Several nicotinic acetylcholine receptor subunits have been detected within cerebellar granule cell layer (GCL), and activation of these receptors may have a significant influence on neuronal synaptic transmission of the cerebellum. The aim of present study was to better understand the roles of nicotinic acetylcholine receptors during the sensory stimulation-evoked synaptic transmission in the cerebellar GCL. Our results showed that cerebellar surface perfusion of nicotine significantly facilitated the cerebellar GCL field potential responses evoked by air-puff stimulation of ipsilateral whisker pad, which exhibited increases in amplitude and area under the curve (AUC) of both stimulus onset responses (N1) and stimulus offset responses (N2). The nicotine-induced increase in AUC of facial stimulation-evoked N1 was dose-dependent with a 50% effective concentration (EC50) of 32.6 μM. Application of either a selective α4β2 nicotinic acetylcholine receptors antagonist, DHβE (1 μM) or a selective α7 nicotinic acetylcholine receptors antagonist, MLA (1 μM) alone attenuated, but not completely abolished the nicotine-induced increases in the amplitude and AUC of the facial stimulation-evoked N1. However, simultaneous blockade of α7 and α4β2 nicotinic acetylcholine receptor subunits abolished the nicotine-induced increase in the amplitude of N1. These results indicate that nicotine activates α7 and α4β2 nicotinic acetylcholine receptor subunits, resulting in an enhancement of facial stimulation-evoked responses in mouse cerebellar GCL. Our results suggest that nicotine modulates the sensory information processing in the cerebellar GCL through α7 and α4β2 subunits nicotinic acetylcholine receptors.