Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins

• Published in: Chinese chemical letters Vol. 21; no. 12; pp. 1487 - 1489
• Main Authors: Wang, Yan, Chai, Zhi Hua, He, Hong Yan, Jiang, Yi Zhe, Fu, Guo Qi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2010; Key Laboratory of Functional Polymer Materials of Ministry of Education,Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China
• Subjects: 3-Aminophenylboronic acid; Hemoglobin; Hydrogels; Lysozyme; Protein imprinting; 分子印迹; 水凝胶; 特异性结合; 聚丙烯酰胺; 蛋白质印迹; 蛋白质相互作用; Protein imprinting; Hemoglobin; 3-Aminophenylboronic acid; Hydrogels; Lysozyme
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2010.06.005

In this letter,N-acryloyl-3-aminophenylboronic acid（AAPBA） was synthesized and then examined as a new functional monomer for protein imprinting.It was allowed to be copolymerized with acrylamide to produce hemoglobin- or lysozyme-imprinted hydrogels.In template rebinding tests,the imprinted gels showed significant increase in the specific binding with the increase of the AAPBA amounts in the prepolymerization recipes.These results indicate that AAPBA may be a useful functional monomer for its moderate interactions with protein molecules.