Histochemical analysis of collagen fibers in giant cell fibroma and inflammatory fibrous hyperplasia

• Published in: Acta histochemica Vol. 118; no. 5; pp. 451 - 455
• Main Authors: Schmidt, Mônica Jarema, Tschoeke, André, Noronha, Lúcia, Moraes, Rafaela Scariot de, Mesquita, Ricardo Alves, Grégio, Ana Maria Trindade, Alanis, Luciana Reis Azevedo, Ignácio, Sérgio Aparecido, Santos, Jean Nunes dos, Lima, Antonio Adilson Soares de, Luiz, Teixeira Suelen, Michels, Arielli Carine, Aguiar, Maria Cássia Ferreira, Johann, Aline Cristina Batista Rodrigues
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.06.2016
• Subjects: Adult; Collagen; Female; Fibrillar Collagens - metabolism; Fibroma; Fibroma - metabolism; Fibroma - pathology; Giant Cell Tumors - metabolism; Giant Cell Tumors - pathology; Giant Cells - metabolism; Giant Cells - pathology; Humans; Hyperplasia - metabolism; Hyperplasia - pathology; Male; Mouth Mucosa - metabolism; Mouth Mucosa - pathology; Mouth Neoplasms - metabolism; Mouth Neoplasms - pathology; Picrosirius red; Collagen; Picrosirius red; Fibroma
• ISSN: 0065-1281; 1618-0372
• DOI: 10.1016/j.acthis.2016.04.007

The aim was to investigate collagen fibers in giant cell fibroma, inflammatory fibrous hyperplasia, and oral normal mucosa. Sixty-six cases were stained with picrosirius red. The slides were observed under polarization, followed by the measurement of the area and the percentage of the type I and type III collagens. The age and gender were obtained from the clinical records. No differences could be observed in both the area and percentage of the type I and type III collagens within the categories of lesions and normal mucosa. In the giant cells fibroma, a greater area and percentage of type I collagen could be identified in individuals of less than 41.5 years (p<0.05). The distribution of type I and type III collagen fibers in the studied lesions followed a similar pattern to that observed in the normal mucosa, indicating a normal collagen maturation process of type III to I. The study supports that multinucleated and stellate cells of the giant cell fibroma appear to be functional within collagen types III and I turnover. The greater amount of type I collagen identified in giant cell fibroma in individuals of less than 41.5 years reinforce the neoplastic nature of lesion.