Central injection of CDP-choline suppresses serum ghrelin levels while increasing serum leptin levels in rats

• Published in: European journal of pharmacology Vol. 764; pp. 264 - 270
• Main Authors: Kiyici, Sinem, Basaran, Nesrin Filiz, Cavun, Sinan, Savci, Vahide
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.10.2015
• Subjects: Animals; Atropine - pharmacology; Blood Glucose - analysis; CDP-choline; Corticosterone - blood; Cytidine Diphosphate Choline - pharmacology; Ghrelin; Ghrelin - blood; Injections; Leptin; Leptin - blood; Male; Mecamylamine - pharmacology; Muscarinic Antagonists - pharmacology; Nicotinic Antagonists - pharmacology; Rats, Wistar; Receptors, Muscarinic - metabolism; Receptors, Nicotinic - metabolism; CDP-choline; Ghrelin; Leptin
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2015.07.014

In this study we aimed to test central administration of CDP-choline on serum ghrelin, leptin, glucose and corticosterone levels in rats. Intracerebroventricular (i.c.v.) 0.5, 1.0 and 2.0µmol CDP-choline and saline were administered to male Wistar-Albino rats. For the measurement of serum leptin and ghrelin levels, blood samples were obtained baseline and at 5, 15, 30, 60 and 120min following i.c.v. CDP-choline injection. Equimolar doses of i.c.v. choline (1.0µmol) and cytidine (1.0µmol) were administered and measurements were repeated throughout the second round of the experiment. Atropine (10µg) and mecamylamine (50µg) were injected intracerebroventricularly prior to CDP-choline and measurements repeated in the third round of the experiment. After 1µmol CDP-choline injection, serum ghrelin levels were suppressed significantly at 60min (P=0.025), whereas serum leptin levels were increased at 60 and 120min (P=0.012 and P=0.017 respectively). CDP-choline injections also induced a dose- and time-dependent increase in serum glucose and corticosterone levels. The effect of choline on serum leptin and ghrelin levels was similar with CDP-choline while no effect was seen with cytidine. Suppression of serum ghrelin levels was eliminated through mecamylamine pretreatment while a rise in leptin was prevented by both atropine and mecamylamine pretreatments. In conclusion; centrally injected CDP-choline suppressed serum ghrelin levels while increasing serum leptin levels. The observed effects following receptor antagonist treatment suggest that nicotinic receptors play a role in suppression of serum ghrelin levels,whereas nicotinic and muscarinic receptors both play a part in the increase of serum leptin levels.