Evolution in Pseudomonas fluorescens

• Published in: Journal of general microbiology Vol. 120; no. 2; pp. 485 - 511
• Main Authors: CHAMPION, ALEX B, BARRETT, ERICKA L, PALLERONI, NORBERTO J, SODERBERG, KATHY L, KUNISAWA, RIYO, CONTOPOULOU, REBECCA, WILSON, ALLAN C, DOUDOROFF, MICHAEL
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.10.1980
• Subjects: Azurin; Biological Evolution; DNA, Bacterial; Genotype; Phenotype; Pseudomonas aeruginosa - classification; Pseudomonas fluorescens; Pseudomonas fluorescens - classification; Pseudomonas fluorescens - genetics
• ISSN: 0022-1287; 1350-0872; 1465-2080
• DOI: 10.1099/00221287-120-2-485

1 Department of Bacteriology and Immunology, University of California, Berkeley, California 94720, U.S.A. 2 Department of Biochemistry, University of California, Berkeley, California 94720, U.S.A. ABSTRACT Summary: The relationships among 93 strains of Pseudomonas fluorescens were investigated by (1) a numerical taxonomic analysis on the results of 150 phenotypic tests, (2) DNA hybridization studies using 16 reference strains, (3) quantitative microcomplement fixation studies using six reference strains with antibodies directed against the protein azurin. In general, the strains fell into distinct clusters. Assignment to these clusters on the basis of azurin immunological similarity showed 98% agreement with assignment based on DNA homology, suggesting that many genes will follow the same pattern. Of the strains that clustered on the basis of genotype (DNA, azurin) 88% also clustered on the basis of phenotype. The occasional non-congruency observed between the genotypic and phenotypic data may be due to the variable rates of phenotypic evolution. These results provide a perspective on the roles of horizontal and vertical transfer of genes in the evolution of this bacterial group. ¶ Department of Biology, University of California, San Diego, La Jolla, California 92093, U.S.A. Research Division, Hoffmann-La Roche Inc., Nutley, New Jersey 07110, U.S.A. Department of Food Science and Technology, University of California, Davis, California 95616, U.S.A. Cutter Laboratories Inc., Fourth and Parker Streets, Berkeley, California 94710, U.S.A.