Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways

• Published in: Phytomedicine (Stuttgart) Vol. 64; p. 153061
• Main Authors: Duangjan, Chatrawee, Rangsinth, Panthakarn, Gu, Xiaojie, Zhang, Shaoxiong, Wink, Michael, Tencomnao, Tewin
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.11.2019
• Subjects: Aging; Animals; Antioxidants; Antioxidants - metabolism; Caenorhabditis elegans; Caenorhabditis elegans Proteins - metabolism; DAF-16/FoxO; DNA-Binding Proteins - metabolism; Flavonoids - analysis; Forkhead Transcription Factors - metabolism; Glochidion zeylanicum; Longevity - drug effects; Malpighiales - chemistry; Oxidative Stress - drug effects; Phenols - analysis; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plants, Medicinal; Reactive Oxygen Species - metabolism; Signal Transduction - drug effects; SKN-1/Nrf2; Transcription Factors - metabolism; GST-4; GLC-MS; DPPH; Juglone; DMSO; EGCG; H2DCF-DA; SKN-1/Nrf2; SKN-1/Nrf-2; Glochidion zeylanicum; Antioxidants; GZD; Caenorhabditis elegans; LC-MS; GZ; GZH; ROS; Aging; SOD-3; DAF-16/FoxO; GZM; ABTS; HPLC; HSP-16.2
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2019.153061

Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported. We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model. The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts. GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans. This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress. [Display omitted]