Ecotoxicological effects of polyethylene microplastics and lead (Pb) on the biomass, activity, and community diversity of soil microbes

• Published in: Environmental research Vol. 252; no. Pt 3; p. 119012
• Main Authors: Song, Xiliang, Li, Changjiang, Qiu, Zhennan, Wang, Chenghui, Zeng, Qiangcheng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2024
• Subjects: Heavy metals; Lead; Microplastic; Soil microbiota; qCO2; FDA; ALP; URE; RDA; MBC; OTU; SD; Pb; ANOVA; Soil microbiota; Lead; HSD; MBN; SR; SUC; MP; AK; NMDS; Microplastic; Heavy metals; AP; LEfSe; SOM; CAT; PVC; PE-MP
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2024.119012

Microplastics and heavy metals are ubiquitous and persistent contaminants that are widely distributed worldwide, yet little is known about the effects of their interaction on soil ecosystems. A soil incubation experiment was conducted to investigate the individual and combined effects of polyethylene microplastics (PE-MPs) and lead (Pb) on soil enzymatic activities, microbial biomass, respiration rate, and community diversity. The results indicate that the presence of PE-MPs notably reduced soil pH and elevated soil Pb bioavailability, potentially exacerbated the combined toxicity on the biogeochemical cycles of soil nutrients, microbial biomass carbon and nitrogen, and the activities of soil urease, sucrase, and alkaline phosphatase. Soil CO2 emissions increased by 7.9% with PE-MPs alone, decreased by 46.3% with single Pb, and reduced by 69.4% with PE-MPs and Pb co-exposure, compared to uncontaminated soils. Specifically, the presence of PE-MPs and Pb, individually and in combination, facilitated the soil metabolic quotient, leading to reduced microbial metabolic efficiency. Moreover, the addition of Pb and PE-MPs modified the composition of the microbial community, leading to the enrichment of specific taxa. Tax4Fun analysis showed the effects of Pb, PE-MPs and their combination on the biogeochemical processes and ecological functions of microbes were mainly by altering amino acid metabolism, carbohydrate metabolism, membrane transport, and signal transduction. These findings offer valuable insights into the ecotoxicological effects of combined PE-MPs and Pb on soil microbial dynamics, reveals key assembly mechanisms and environmental drivers, and highlights the potential threat of MPs and heavy metals to the multifunctionality of soil ecosystems. [Display omitted] •PE-MPs reduce soil pH and enhance Pb bioavailability.•PE-MP-Pb suppresses CO2 emissions and lowers microbial efficiency.•Actinobacteriota, Proteobacteria, Chloroflexi, and Firmicutes are key contributors in Pb and PE-MPs coexisting soils.•SOM, AP, and Pb levels are the primary factors influencing bacterial communities.•Amino acid, carbohydrate metabolism, membrane transport regulate microbial community.