Roles of oxidative DNA damage of bone marrow hematopoietic cells in steroid-induced avascular necrosis of femoral head

• Published in: Genetics and molecular research Vol. 15; no. 1
• Main Authors: Zhao, Z Q, Bai, R, Liu, W L, Feng, W, Zhao, A Q, Wang, Y, Wang, W X, Sun, L, Wu, L S, Cui, S X
• Format: Journal Article
• Language: English
• Published: Brazil 24.03.2016
• Subjects: Animals; DNA Damage; Endotoxins - toxicity; Escherichia coli; Female; Femur - metabolism; Femur - pathology; Hematopoietic Stem Cells - metabolism; Male; Osteonecrosis - etiology; Osteonecrosis - genetics; Osteonecrosis - metabolism; Osteonecrosis - pathology; Oxidative Stress; Rabbits; Steroids - toxicity
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/gmr.15017706

An animal model of steroid-induced avascular necrosis of femoral head (SANFH) was established to investigate the role of oxidative DNA damage of bone marrow hematopoietic cells in SANFH. Forty-five-month-old Japanese white rabbits (male or female, 2.5 ± 0.5 kg) were randomly divided into groups A (methylprednisolone + Escherichia coli endotoxin), B (methylprednisolone alone), C (E. coli endotoxin alone), and D (blank control). The animals were sacrificed two and four weeks after administration of the last dose (N = 5 each group and each time). Left and right femoral heads were fixed and decalcified. Empty lacunae were counted by hematoxylin and eosin staining and oxidative DNA damage of bone marrow hematopoietic cells was detected by immunohistochemistry. At week 2, the rate of oxidative DNA damage in bone marrow hematopoietic cells was significantly higher in group A than in groups B, C, and D (P < 0.01), while there was no significant difference between groups B, C, and D. At week 4, the rate of oxidative DNA damage in bone marrow hematopoietic cells was significantly higher in group A than in groups B, C, and D (P < 0.01), while there was no significant difference among groups B, C, and D. Thus, oxidative DNA damage of bone marrow hematopoietic cells appears to play an important role in SANFH.