Banding and FISH in three species of Vernonia, subsection Macrocephalae (Asteraceae, Vernonieae)

• Published in: Plant systematics and evolution Vol. 298; no. 5; pp. 969 - 974
• Main Authors: de Oliveira, V. M, Semir, J, Forni Martins, E. R
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 01.05.2012; Springer Vienna; Springer Nature B.V
• Subjects: Biological taxonomies; Biomedical and Life Sciences; Chromosome banding; Chromosomes; Evolution; Fish; Fluorescence; Fluorescence in situ hybridization; Fluorophores; Genera; Karyotypes; karyotyping; Life Sciences; Nucleolus organizer region; Original Article; Plant Anatomy/Development; Plant Ecology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Plants; Ploidies; Ribosomal DNA; savannas; Species; Vegetation; Vernonia; Brazil; rDNA; Chromosome; Mitosis; Cytogenetic
• ISSN: 0378-2697; 1615-6110; 2199-6881
• DOI: 10.1007/s00606-012-0606-8

To complement our knowledge about the karyotypes of the genus Vernonia Schreb., different techniques of chromosome banding, including AgNOR, triple staining with fluorochromes CMA/DA/DAPI (CDD), and fluorescence in situ hybridization (FISH) for the 45S rDNA probe, were applied to three species of subsection Macrocephalae. Vernonia bardanoides was collected from an area of cerrado (savanna) vegetation in Itirapina, São Paulo State, Brazil, and V. linearifolia and V. tomentella were collected from areas of rocky, open altitudinal vegetation in Joaquim Felicio and Diamantina, respectively, in Minas Gerais State. All species showed two terminal CMA+ and NOR bands. FISH indicated two terminal 45S rDNA sites in V. linearifolia and V. tomentella, and six in V. bardanoides.