Puerarin promoted proliferation and differentiation of dopamine-producing cells in Parkinson’s animal models

• Published in: Biomedicine & pharmacotherapy Vol. 106; pp. 1236 - 1242
• Main Authors: Shiying, Liu, Xinhui, Qu, Guanghua, Jin, Feng, Nie, Feng, Liu, Shumei, Chen, Fan, Hu
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.10.2018
• Subjects: Animals; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Corpus Striatum - metabolism; Corpus Striatum - pathology; Corpus Striatum - surgery; DA neuron; Disease Models, Animal; Dopamine - metabolism; Dopamine Plasma Membrane Transport Proteins - genetics; Dopamine Plasma Membrane Transport Proteins - metabolism; Dopaminergic Neurons - drug effects; Dopaminergic Neurons - metabolism; Dopaminergic Neurons - pathology; Dopaminergic Neurons - transplantation; Dose-Response Relationship, Drug; Isoflavones - pharmacology; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; Mesenchymal Stem Cells - pathology; Neuroprotective Agents - pharmacology; Oxidopamine; Parkinsonian Disorders - chemically induced; Parkinsonian Disorders - metabolism; Parkinsonian Disorders - pathology; Parkinsonian Disorders - surgery; Parkinson’s disease; Puerarin; Rats, Sprague-Dawley; Signal Transduction - drug effects; Stem cell transplantation; Time Factors; Tubulin - genetics; Tubulin - metabolism; Tyrosine 3-Monooxygenase - genetics; Tyrosine 3-Monooxygenase - metabolism; Stem cell transplantation; DA neuron; Puerarin; Parkinson’s disease; Mesenchymal stem cells
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2018.07.058

[Display omitted] •In vitro, PR promotes the differentiation of TH+ cells from MSCs.•In vivo, PR promotes the survival of transplantated DA-producing cells.•In vivo, PR promoted the transplanted cells proliferated and differentiated into TH + cells. Parkinson’s disease (PD) is caused by the gradual loss of dopamine-producing cells in the brain. This study evaluated the potential neuroprotective role of puerarin (PR) on dopamine (DA)-producing cells in vitro and in vivo. In vitro, the effects of PR on proliferation and differentiation and DA releases of mesenchymal stem cells (MSCs) were assayed by CCK-8, flow cytometry, real-time PCR and ELISA respectively. Then the differentiated cells were labeled with enhanced green fluorescent protein (EGFP) and administrated into PD animal models induced by 6-OHDA. The proliferation and differentiation of labeled cells were identified by fluorescence microscopy and immunostaining. In vitro, after being treated with different concentrations of PR for 1 week, the TUJ1, TH and DAT protein and mRNA expression and DA releases increased significantly. In vivo, after transplantation of PR-treated DA-producing cells, the symptoms of PD improved significantly from the second week after transplantation; more transplanted cells survived and migrated to wider region along injection line; more transplanted cells proliferated and differentiated into TH+ cells; more DA was detected in the striatum during 6 weeks’ observation. The results suggest that PR promote DA neuron survival, proliferation and differentiation.