Nucleic acid-based fluorescent methods for the determination of DNA repair enzyme activities: A review

• Published in: Analytica chimica acta Vol. 1060; pp. 30 - 44
• Main Authors: Lee, Chang Yeol, Kim, Hansol, Park, Ki Soo, Park, Hyun Gyu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 04.07.2019; Elsevier BV
• Subjects: Analytical methods; Deoxyribonucleic acid; Disease; DNA; DNA - chemistry; DNA damage; DNA Repair; DNA repair enzyme; DNA Repair Enzymes - analysis; DNA Repair Enzymes - metabolism; Drug development; Enzymatic activity; Enzymes; Fluorescence; Fluorescent biosensor; Inhibitor; Nucleic acid; Outbreaks; Repair; Reviews; Fluorescent biosensor; DNA repair enzyme; Inhibitor; Disease; DNA damage; Nucleic acid
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2018.12.055

DNA repair pathways are closely associated with the maintenance of genomic integrity, disease outbreak, and the development of therapeutics. Owing to these significances, novel analytical methods for enzymes that are involved in the DNA repair pathways have been actively investigated. This review focuses on discussions on nucleic acid-based methods, especially those based on the fluorescence, for the determination of DNA repair enzymes. Furthermore, this review not only provides meaningful insights in creating ingenious fluorescent detection methods but it also suggests future directions that will be followed in developing new analytical technologies for the DNA repair enzymes. [Display omitted] •Nucleic acid-based fluorescent methods for assaying DNA repair enzymes, are summarized.•Novel assays which utilize functional nucleic acids, are discussed.•Ultra-sensitive strategies that rely on isothermal nucleic acid amplification, are discussed.•Future directions in the area of fluorescent enzyme assays are suggested.