In-Vitro Cytotoxicity and Cell Cycle Analysis of Two Novel bis-1,2, 4-triazole derivatives: 1,4-bis[5-(5-mercapto-1,3,4-oxadiazol-2-yl-methyl)-thio-4-(p-tolyl)-1,2,4-triazol-3-yl]-butane (MNP-14) and 1,4-bis[5-(carbethoxy-methyl)-thio-4-(p-ethoxy phenyl)-1,2,4-triazol-3-yl]-butane (MNP-16)

• Published in: Nucleosides, nucleotides & nucleic acids Vol. 30; no. 11; pp. 873 - 885
• Main Authors: Purohit, Madhusudan N., Panjamurthy, Kuppusamy, Elango, Santhini, Hebbar, Karteek, Mayur, Yergeri C., Raghavan, Sathees C.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis Group 01.11.2011
• Subjects: Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; bis-1, 2, 4-triazole; Butanes - chemistry; Butanes - pharmacology; Cell Cycle - drug effects; cell cycle analysis; Cell Line, Tumor; Cytotoxicity; DNA damage; Humans; Lung Neoplasms - drug therapy; MTT assay; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy; Triazoles - chemistry; Triazoles - pharmacology
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1080/15257770.2011.608395

In the present study, we have tested the cytotoxic and DNA damage activity of two novel bis-1,2,4 triazole derivatives, namely 1,4-bis[5-(5-mercapto-1,3,4-oxadiazol-2-yl-methyl)-thio-4-(p-tolyl)-1,2,4-triazol-3-yl]-butane (MNP-14) and 1,4-bis[5-(carbethoxy-methyl)-thio-4-(p-ethoxy phenyl) -1,2,4-triazol-3-yl]-butane (MNP-16). The effect of these molecules on cellular apoptosis was also determined. The in-vitro cytotoxicity was evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay as well as Trypan blue dye exclusion methods against human acute lymphoblastic leukemia (MOLT4) and lung cancer cells (A549). Our results showed that MNP-16 induced significant cytotoxicity (IC 50 of 3-5 μM) compared with MNP-14. The cytotoxicity induced by MNP-16 was time and concentration dependent. The cell cycle analysis by flow cytometry (fluorescence-activated cell sorting [FACS]) revealed that though there was a significant increase in the apoptotic population (sub-G 1 phase) with an increased concentration of MNP-14 and 16, there was no cell cycle arrest. Further, the comet assay results indicated considerable DNA strand breaks upon exposure to these compounds, thereby suggesting the possible mechanism of cytotoxicity induced by MNP-16. Hence, we have identified a novel molecule (MNP-16) which could be of great clinical relevance in cancer therapeutics.