Effects of nitric oxide on nitrogen metabolism and the salt resistance of rice (Oryza sativa L.) seedlings with different salt tolerances

• Published in: Plant physiology and biochemistry Vol. 155; pp. 374 - 383
• Main Authors: Huang, Jie, Zhu, Chunquan, Hussain, Sajid, Huang, Jing, Liang, Qingduo, Zhu, Lianfeng, Cao, Xiaochuang, Kong, Yali, Li, Yefeng, Wang, Liping, Li, Jianwu, Zhang, Junhua
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.10.2020
• Subjects: Nitric oxide; Nitric Oxide - metabolism; Nitrogen - metabolism; Oryza - physiology; Rice; Salt resistance; Salt Tolerance; Seedlings - physiology; Sodium Chloride; Sucrose metabolism; Sucrose metabolism; Nitrogen metabolism; Salt resistance; Nitric oxide; Rice
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.06.013

Salt stress inhibits rice productivity seriously. Nitric oxide (NO) is an endogenous signaling molecule in plants that can improve the resistance of rice to abiotic stresses. Previous studies also showed that nitrogen metabolism is essential for rice stress-tolerance. However, the physiological and molecular mechanisms by how NO affects the nitrogen metabolisms of rice seedlings remain unclear. A hydroponic experiment with two rice varieties, Jinyuan85 (salt tolerant) and Liaojing763 (salt sensitive), was carried out to explore whether NO could alleviate the negative effects of salt stress on nitrogen metabolism and increase salt resistance of rice seedlings. The results showed that (1) the application of NO alleviated the inhibitory effects of salt stress on plant height and biomass accumulation, and increased the nitrogen content of rice leaf. (2) the accumulation of the sucrose and proline was markedly increased in salt stress after application of NO, and peroxidase activities was increased by 107% and 67.7% for Jinyuan85 and Liaojing763, respectively. (3) NO significantly increased the activities of glutamate dehydrogenase, sucrose synthase and sucrose phosphate synthase in both rice varieties under salt stress. (4) Additionally, NO regulated the expression levels of AMT, NIA and SUT genes, but these regulation effects are different with rice varieties and treatments. The results suggested that NO mainly increased the glutamate dehydrogenase and peroxidase activities and sucrose accumulation to enhance the nitrogen metabolism and antioxidative capacity, and alleviated the negative effects of salt stress on rice performance. •Application of nitric oxide (NO) alleviated the salt stress in rice seedlings.•NO application improved the activity of glutamate dehydrogenase enzymes.•NO application promoted the accumulation of sucrose and proline in rice leaf.