Effect of hydroxyamines derived from lapachol and norlachol against Staphylococcus aureus strains carrying the NorA efflux pump

• Published in: Infection, genetics and evolution Vol. 84; p. 104370
• Main Authors: Figueredo, Fernando Gomes, Ramos, Ingrid T.L., Paz, Josinete A., Silva, Tania M.S., Câmara, Celso A., de Morais Oliveira-Tintino, Cícera Datiane, Tintino, Saulo Relison, de Farias, Pablo Antônio Maia, Menezes, Irwin Rose Alencar de, Coutinho, Henrique Douglas Melo, Fonteles, Marta Maria de F.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2020
• Subjects: Antibiotics; Efflux pump inhibitor; Lapachol; Norlachol; Resistance; Resistance; Lapachol; Efflux pump inhibitor; Norlachol; Antibiotics
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2020.104370

Isolated substances and those organically synthesized have stood out over the years for their therapeutic properties, including their antibacterial activity. These compounds may be an alternative to the production of new antibiotics or may have the ability to potentiate the action of preexisting ones. In this context, the objective of this study was to evaluate the in vitro antibacterial and efflux pump inhibitory activity of hydroxyamines derived from lapachol and norlachol, more specifically the compounds 2-(2-Hydroxyethylamino)-3-(3-methyl-2-butenyl)-1,4 dihydro-1,4-naphthalenedione, 2-(2-Hydroxyethylamino)-3-(2-methyl-propenyl)[1,4]naphthoquinone and 2-(3-Hydroxypropylamino)-3-(3-methyl-2-butenyl)-[1,4]naphthoquinone, against Staphylococcus aureus strains carrying the NorA efflux pump mechanism. The substances were synthesized from 2-hydroxy-quinones, lapachol and nor-lapachol, obtaining the corresponding 2-methoxylated derivatives via dimethyl sulfate alkylation in a basic medium, which then reacted chemoselectively with 2-ethanolamine and 3-propanolamine to form the corresponding amino alcohols. All three molecules underwent a virtual structure-based analysis (docking). The antibacterial activity of the substances was measured by determining their Minimum Inhibitory Concentration (MIC) and a microdilution assay was performed to verify efflux pump inhibition using the substances at a sub-inhibitory concentration. The results were subjected to statistical analysis using an analysis of variance (ANOVA) followed by Bonferroni's post hoc test. The substances obtained MIC values ≥1024 μg/mL, however, a significant reduction of their MICs was observed when the substances were associated with norfloxacin and ethidium bromide, with this effect being attributed to efflux pump inhibition. Following a virtual analysis based on its structure (docking), information regarding the affinity of new ligands for the ABC efflux pump were observed, thus contributing to the understanding of their mechanism of molecular interactions and the discovery of functional ligands associated with a reduction in bacterial resistance. •Compounds 3, 4 and 5 do not present a direct antibacterial activity.•These substances reduced the MIC of norfloxacin when associated.•These substances reduced the MIC of ethidium bromide when associated.