Molecular characterisation of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from hospital and ambulatory patients in Germany

• Published in: Veterinary microbiology Vol. 200; pp. 130 - 137
• Main Authors: Pietsch, Michael, Eller, Christoph, Wendt, Constanze, Holfelder, Martin, Falgenhauer, Linda, Fruth, Angelika, Grössl, Tobias, Leistner, Rasmus, Valenza, Giuseppe, Werner, Guido, Pfeifer, Yvonne
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: Ambulatory Care Facilities; Animals; Bacterial Typing Techniques; beta-Lactamases - genetics; CTX-M-15; Drug Resistance, Multiple - genetics; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Genotype; Germany - epidemiology; Hospitals; Humans; Molecular Epidemiology; Multidrug-resistance; Multilocus Sequence Typing; Nosocomial infection; Phylogeny; Phylogroup B2; Prevalence; ST131; CTX-M-15; Nosocomial infection; ST131; Multidrug-resistance; Phylogroup B2
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2015.11.028

•Molecular analyses of 233 3rd gen. cephalosporin-resistant E.coli from Germany.•Most prevalent ESBLs were CTX-M-15 (50.4%), CTX-M-1 (28.4%) and CTX-M-14 (5.6%).•High rates of co-resistance to ciprofloxacin (72.5%).•E. coli-ST131 was the most common sequence type (35.8%).•Further more prevalent STs were ST410, ST10 and ST38. The increase of Escherichia coli producing extended-spectrum β-lactamases (ESBL) in hospitals and their emergence as intestinal colonisers of healthy humans is of concern. Transmission ways and the extent of spread of distinct E. coli clones or ESBL genes among humans and animals via the food chain or the environment is a matter of debate. In this study we determined ESBL genotypes in E. coli isolates (n=233) resistant to 3rd generation cephalosporins from hospitals and medical practices using PCR and sequencing. Bacterial strain typing was performed by PCR-based phylogrouping, multilocus sequence typing (MLST) and a ST131-specific PCR. Results showed that CTX-M-15 (50.4%), CTX-M-1 (28.4%) and CTX-M-14 (5.6%) were the most common ESBL types. Especially, CTX-M-15 was associated with E. coli ST131 of phylogenetic group B2, which was the dominant sequence type among our isolates (35.8%). MLST typing revealed 40 different sequence types (STs), with ST131, ST410, ST10 and ST38 as the most prevalent ones. Our findings give an overview of the current distribution of ESBL-producing E. coli isolates from humans in Germany. E. coli O25b:H4-ST131 was confirmed to be the most common clone, which is known for its successful dissemination worldwide. Although heterogeneity among the isolates was found, several successful clones previously described in animals (ST410, ST10) also occurred in our isolate collection. Further detailed investigations of ESBL-producing isolates from different habitats are needed to evaluate possible transfer ways.