Antifungal activity of essential oils extract from Origanum floribundum Munby, Rosmarinus officinalis L. and Thymus ciliatus Desf. against Candida albicans isolated from bovine clinical mastitis

• Published in: Journal de mycologie médicale Vol. 27; no. 2; pp. 245 - 249
• Main Authors: Ksouri, S., Djebir, S., Bentorki, A.A., Gouri, A., Hadef, Y., Benakhla, A.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.06.2017
• Subjects: Animals; Antifungal activity; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; Bovine mycotic mastitis; Candida albicans - drug effects; Candida albicans - growth & development; Candida albicans - isolation & purification; Candida albicans; Candidiasis - microbiology; Candidiasis - veterinary; Cattle; Essential oil; Female; Mastitis, Bovine - microbiology; Microbial Sensitivity Tests; Oils, Volatile - isolation & purification; Oils, Volatile - pharmacology; Origanum - chemistry; Plant Extracts - pharmacology; Plant Oils - isolation & purification; Plant Oils - pharmacology; Rosmarinus - chemistry; Thymus Plant - chemistry; Candida albicans; Antifungal activity; Essential oil; Bovine mycotic mastitis
• ISSN: 1156-5233; 1773-0449
• DOI: 10.1016/j.mycmed.2017.03.004

The aim of this study is to limit the antibiotic use in mastitis treatment and to find other alternatives. The antifungal activity of the essential oils from Origanum floribundum Munby., Rosmarinus officinalis L. and Thymus ciliatus Desf. is studied in the present work against a Candida albicans reference strain and ten C. albicans isolated strains from bovine clinical mastitis. Essential oils were extracted by hydrodistillation technique using Clevenger apparatus. Their chromatographic analysis was performed with a Gas Chromatograph/Mass Spectrometer (GC/MS). Antifungal activities of essential oils were investigated by macrobroth method of dilution in tubes to determine the Minimum Inhibitory Concentrations (MIC 80%). Analysis of the essential oil showed chemical profile dominated by thymol (50.47 and 62.41%) and P-cymene (24.22 and 15.51%) in the oregano and the thyme respectively, 1, 8-cineol (31.50%) and α-pinene (18.33%) in Rosemary. The three essential oils revealed highly effective anticandidal activity, with an MIC of 80% values ranged from 15.02 to 31.08μg/mL. These results suggest that essential oils studied can be real alternatives in the control of mastitis fungi but deserving studies more in-depth and detailed on their application in vivo.