Toxicological effect of endocrine disrupting heavy metal (Pb) on Mekong silurid Pangasius catfish, Pangasius hypophthalmus

• Published in: Environmental research Vol. 231; no. Pt 1; p. 116033
• Main Authors: Kumar Reddy, C. Prabhanjan, Manikandavelu, D., Arisekar, Ulaganathan, Albeshr, Mohammed F., Alrefaei, Abdulwahed Fahad, Sudhakar, O., Keerthana, M., Packialakshmi, J. Saranya
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.08.2023
• Subjects: Animals; Antioxidants - pharmacology; Catfishes; Enzymatic activity; Gills; Hematology; Histopathology; Lead; Lead - toxicity; Liver; Metals, Heavy - toxicity; P. hypophthalmus; Toxicological marker; Water Pollutants, Chemical - toxicity; P. hypophthalmus; Lead; Histopathology; Enzymatic activity; Hematology; Toxicological marker
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2023.116033

The current study aimed to investigate the effects of lead nitrate exposure on the enzymatical, haematological, and histological changes in the gill, liver, and kidney of Pangasius hypophthalmus. The fish were divided into six groups and treated with different Pb concentrations. The LC50 value of Pb was 55.57 mg/L at 96 h for P. hypophthalmus, and sublethal toxicity was assessed for 45 days at 1/5th (11.47 mg/L) and 1/10th (5.57 mg/L) of LC50 concentration. Enzymes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, alkaline phosphate (ALP), and lactate dehydrogenase (LDH) content increased significantly during sublethal toxicity of Pb. The reduction of HCT and PCV indicates an anemic condition due to the toxicity of Pb. Differential leucocytes, lymphocytes, and monocytes and their % values significantly decreased, indicating Pb exposure. The main histological changes observed in the gills were the destruction of secondary lamellae, the fusion of adjacent gill lamellae, primary lamellae hypertrophy, and severe hyperplasia, while in kidney exposed to Pb showed melanomacrophages, increased periglomerular, peritubular space, vacuolation, shrunken glomerulus, destruction of tubular epithelium, and hypertrophy of distal convoluted segment. The liver showed severe necrosis and rupture of hepatic cells, hyper trepheoid bile duct, shifting of nuclei, and vascular hemorrhage, while in the brain, binucleus, mesoglea cells, vacuole, and ruptured nucleus were observed. In conclusion, P. hypophthalmus, which has been exposed to Pb has developed a number of toxicity markers. Consequently, prolonged exposure to higher Pb concentrations may be harmful to fish health. The findings strongly suggest that the lead had a detrimental impact on the P. hypophthalmus population, as well as on the water quality and non-target aquatic organisms. •LC50 value of lead (Pb) was 55.57 ppm at 96 h for P. hypophthalmus.•RBC and WBC count increased significantly at a sub-lethal concentration of Pb.•Fish organs showed toxicological signs due to Pb toxicity.•Pb sublethal toxicity dramatically raised AST, ALT, ALP, and LDH levels.•Pb alters the mechanism of aquatic organisms thus, is more toxic to the environment.