LED lamps enhance somatic embryo maturation in association with the differential accumulation of proteins in the Carica papaya L. ‘Golden’ embryogenic callus

• Published in: Plant physiology and biochemistry Vol. 143; pp. 109 - 118
• Main Authors: Almeida, Felipe Astolpho, Vale, Ellen Moura, Reis, Ricardo Souza, Santa-Catarina, Claudete, Silveira, Vanildo
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.10.2019
• Subjects: Carica - genetics; Carica - metabolism; Carica papaya L; Clonal propagation; Comparative proteomics; Indoleacetic Acids - metabolism; Label-free proteomics; LED; Light-emitting diode; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Somatic Embryogenesis Techniques; Proteomics - methods; Somatic embryogenesis; Somatic embryogenesis; LED; Comparative proteomics; Clonal propagation; Light-emitting diode; Carica papaya L; Label-free proteomics
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2019.08.029

The use of light-emitting diode (LED) lamps has been shown to be a promising approach for improving somatic embryo maturation during somatic embryogenesis. The aim of this work was to study the influence of the light source on somatic embryo differentiation and its relationship with the differential abundance of proteins in the Carica papaya L. ‘Golden’ embryogenic callus at 14 days of maturation. The white plus medium-blue (WmB) LED and fluorescent lamp treatments produced an average of 82.4 and 47.6 cotyledonary somatic embryos per callus, respectively. A shotgun proteomics analysis revealed 28 upaccumulated and 7 downaccumulated proteins. The proteins upaccumulated in the embryogenic callus matured under the WmB LED lamp compared with that matured under the fluorescent lamp included indole-3-acetic acid-amido synthetase (GH3) and actin-depolymerizing factor 2 (ADF2), which are involved in the regulation of auxin levels by auxin conjugation and transport. Additionally, proteins related to energy production (aconitate, ADH1, GAPCp, PKp and TPI), cell wall remodeling (PG and GLPs), and intracellular trafficking (NUP50A, IST1, small GTPases and H+-PPase) showed significantly higher abundance in the embryogenic callus incubated under the WmB LED lamp than in that incubated under the fluorescent lamp. The results showed that the WmB LED lamp improved somatic embryo maturation in association with the differential accumulation of proteins in the C. papaya ‘Golden’ embryogenic callus. [Display omitted] •The WmB use of LED lamps is more suitable than fluorescent lamps, promoting the maturation of C. papaya somatic embryos.•Callus matured under the WmB LED compared with fluorescent lamps showed a differential accumulation of proteins.•WmB LED induced a differential regulation of proteins related to the control of auxin levels and energy production.