Effects of Zizyphus lotus L. (Desf.) polyphenols on Jurkat cell signaling and proliferation

• Published in: International immunopharmacology Vol. 15; no. 2; pp. 364 - 371
• Main Authors: Abdoul-Azize, Souleymane, Bendahmane, Malika, Hichami, Aziz, Dramane, Gado, Simonin, Anne-Marie, Benammar, Chahid, Sadou, Hassimi, Akpona, Simon, El Boustani, Es-Saddik, Khan, Naim A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2013
• Subjects: Calcium; Calcium Signaling - drug effects; Cell cycle; Cell Proliferation - drug effects; Extracellular Signal-Regulated MAP Kinases - metabolism; Fruit - chemistry; Gene Expression Regulation; Humans; IL-2; Immunosuppression; Inflammation - immunology; Interleukin-2 - genetics; Interleukin-2 - metabolism; Intracellular pH; Jurkat Cells; Jurkat T-cells; Polyphenols - pharmacology; RNA, Messenger - analysis; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Thapsigargin - immunology; Ziziphus - chemistry; Zizyphus lotus L. (Desf.) polyphenols; [Ca2+]i; Calcium; PHA; Zizyphus lotus L. (Desf.) polyphenols; IL-2; Jurkat T-cells; MAPK; pHi; Intracellular pH; PA; TG; Cell cycle; ZLP
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2012.11.013

We assessed the effects of Zizyphus lotus L. (Desf.) polyphenols (ZLP) on T-cell signaling and proliferation. Our results showed that ZLP exerted no effect on the increases in intracellular free calcium concentrations, [Ca2+]i, in human Jurkat T-cells. However, ZLP modulated the thapsigargin-induced increases in [Ca2+]i in these cells. ZLP treatment was found to decrease the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). In addition, ZLP induced a rapid (t1/2=33s) and dose-dependent decrease in intracellular pH (pHi) in human Jurkat T-cells. Furthermore, ZLP significantly curtailed T-cell proliferation by diminishing their progression from S to G2/M phase of cell cycle, and the expression of interleukin-2 (IL-2) mRNA. Taken together, the results of the present study demonstrate that ZLP modulate cell signaling and exert immunosuppressive effects in human T-cells. ► Zizyphus lotus polyphenols (ZLP) modulate Jurkat cell activation at the molecular level. ► The ZLP altered increases in [Ca2+]i, evoked by thapsigargin. ► The ZLP also induced a decrease in the intracellular pH in Jurkat cells. ► The ZLP blocked T-cell cycle progression and decreased MAP kinase phosphorylation.