Rapid liquid chromatography tandem mass spectrometry method for targeted quantitation of human performance metabolites in saliva

•Targeted method for quantitating eight analytes of human performance in saliva.•Simple sample preparation procedure allows for nearly 100% analyte recovery.•Rapid liquid chromatography run is ideal for high throughput.•Comparison of baseline shows very little difference due to occupational lifestyl...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 1601; pp. 205 - 213
Main Authors McBride, Ethan M., Lawrence, Richard J., McGee, Kirstin, Mach, Phillip M., Demond, Paul S., Busch, Michael W., Ramsay, John W., Hussey, Erika K., Glaros, Trevor, Dhummakupt, Elizabeth S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 13.09.2019
Subjects
Human performance
Inflammation
Metabolomics
Saliva
Soldier
Stress
Metabolomics
Soldier
Inflammation
Saliva
Human performance
Stress
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Summary:•Targeted method for quantitating eight analytes of human performance in saliva.•Simple sample preparation procedure allows for nearly 100% analyte recovery.•Rapid liquid chromatography run is ideal for high throughput.•Comparison of baseline shows very little difference due to occupational lifestyle. Saliva is increasingly being targeted for metabolic studies due to its non-invasive collection methods. Tracing levels of certain metabolites within biofluids can provide indications for a myriad of physiological conditions. This study was performed on a panel of eight analytes found in saliva that have shown associations with physiological conditions of human performance, such as stress, inflammation, and circadian rhythm. This dual polarity liquid chromatography tandem mass spectrometric (LCMS/MS) method was developed to accommodate a diverse group of analytes including steroids, alkaloids, and neurotransmitters. Samples collected during field exercises from soldiers were compared to those of civilians and baseline levels of each of these compounds was determined in saliva. Although most analytes showed no significant differences between the two populations, relative cortisol levels were higher for soldiers than for civilians. This developed dual polarity LCMS/MS method can be applied to very diverse groups of salivary analytes simultaneously.
ISSN:0021-9673
1873-3778
DOI:10.1016/j.chroma.2019.04.071