Evaluation of the antifungal and biochemical activities of mefentrifluconazole against Botrytis cinerea

• Published in: Pesticide biochemistry and physiology Vol. 173; p. 104784
• Main Authors: Li, Tongtong, Li, Haolin, Liu, Tingting, Zhu, Jiamei, Zhang, Lanyun, Mu, Wei, Liu, Feng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2021
• Subjects: Antifungal Agents - pharmacology; Biochemical; Botrytis; Botrytis cinerea; Control efficacy; Fluconazole - analogs & derivatives; Fungicides, Industrial - pharmacology; Mefentrifluconazole; Plant Diseases; Sensitivity; Sensitivity; Biochemical; Control efficacy; Botrytis cinerea; Mefentrifluconazole
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2021.104784

Mefentrifluconazole is the first product of a new sub-class of triazoles fungicides, i.e., the isopropanol triazoles, with the broad spectrum and high activity. In this study, the potential and biochemical activities of mefentrifluconazole against Botrytis cinerea were investigated. The frequency distribution of all EC50 values of mefentrifluconazole against mycelial growth and germ tube elongation of 106 isolates formed unimodal curve, with the mean EC50 values of 0.124 ± 0.025 and 0.015 ± 0.008 μg/mL, respectively. The effect of mefentrifluconazole against gray mold was determined on detached leaves of cucumber in vivo, the treatment of mefentrifluconazole at 200 μg/mL provided 100% preventative efficacy and 72.7% curative efficacy. No evident correlation was detected between the sensitivity of B. cinerea to mefentrifluconazole and that to tebuconazole, difenoconazole, myclobutanil, hexaconazole, triadimefon, flusilazole and pyrisoxazole (P > 0.05). Mefentrifluconazole treatment resulted in the increase of mycelium branch, the decrease of ergosterol content and the changes of the permeability of cell membrane. These studies evaluated the potential of mefentrifluconazole to control gray mold and helped us to understand the possible biochemical activity of mefentrifluconazole against B.cinerea. [Display omitted] •Baseline sensitivity of B. cinerea to mefentrifluconazole was established.•Mefentrifluconazole provide excellent protective and curative efficacy against B. cinerea on detached leaves of cucumber.•There was no evident cross-sensitivity between mefentrifluconazole and other seven DMI fungicides (P>0.05).•Mefentrifluconazole affected the normal development of B. cinerea with increased branches of mycelia.•Mefentrifluconazole influenced membrane permeability and content of ergosterol of B. cinerea.