Reliable diagnosis of Trichosomoides crassicauda in the urinary bladder of the rat

Two reliable methods are described for identifying infection of laboratory rats with the nematode Trichosomoides crassicauda. The first is a rapid method where cryostat sections of the rat urinary bladder are stained with acridine orange and viewed under a fluorescence microscope. The second involve...

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Bibliographic Details
Published inLaboratory animals (London) Vol. 22; no. 2; pp. 162 - 165
Main Authors Cornish, J, Vanderwee, M.A, Findon, G, Miller, T.E
Format Journal Article
LanguageEnglish
Published London, England SAGE Publications 01.04.1988
Subjects
Animals
bladder
disease diagnosis
Female
fluorescence microscopy
Male
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Nematoda - isolation & purification
Nematoda - ultrastructure
nematode infections
Nematode Infections - diagnosis
Nematode Infections - veterinary
rats
Rats - parasitology
Rodent Diseases - diagnosis
scanning electron microscopy
Urinary Bladder - parasitology
Urinary Bladder - ultrastructure
Urinary Bladder Diseases - diagnosis
Urinary Bladder Diseases - veterinary
Urine - parasitology
RATS
URINARY BLADDER
FLUORESCENCE MICROSCOPY
SCANNING ELECTRON MICROSCOPY
NEMATODE
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Summary:Two reliable methods are described for identifying infection of laboratory rats with the nematode Trichosomoides crassicauda. The first is a rapid method where cryostat sections of the rat urinary bladder are stained with acridine orange and viewed under a fluorescence microscope. The second involves the stabilization of the bladder surface prior to examination using scanning electron microscopy (SEM).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0023-6772
1758-1117
DOI:10.1258/002367788780864385