Impact of high irradiance and UV-B on the photosynthetic activity, pro-/antioxidant balance and expression of light-activated genes in Arabidopsis thaliana hy4 mutants grown under blue light

• Published in: Plant physiology and biochemistry Vol. 167; pp. 153 - 162
• Main Authors: Kreslavski, Vladimir D., Khudyakova, Aleksandra Yu, Strokina, Valeria V., Shirshikova, Galina N., Pashkovskiy, Pavel P., Balakhnina, Tamara I., Kosobryukhov, Anatoly A., Kuznetsov, Vladimir V., Allakhverdiev, Suleyman I.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.10.2021
• Subjects: Antioxidants; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - radiation effects; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Ascorbate Peroxidases - metabolism; Cryptochrome 1; Gene expression; Gene Expression Regulation, Plant; Peroxidase - genetics; Peroxidase - metabolism; Photosynthesis; Photosystem II; Pro-/antioxidant balance; Reactive Oxygen Species; Resistance to high irradiance; Resistance to UV-B; Ultraviolet Rays; Chl; PSII; UAPs; APX; Photosystem II; BL; HIL; TBARs; Cry; Gene expression; GPX; Arabidopsis thaliana; PA; HBL; Resistance to high irradiance; MBL; Resistance to UV-B; Pro-/antioxidant balance; Cryptochrome 1; WT
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2021.07.030

The impacts of high-intensity light (HIL) (4 h) and UV-B radiation (1 h) on the photosynthetic activity, content of photosynthetic and UV-absorbing pigments (UAPs), activity of antioxidant enzymes (ascorbate peroxidase (APX) and guaiacol-dependent peroxidase (GPX)), content of thiobarbituric acid reactive substances (TBARs), expression of some light-regulated genes in 25-day-old wild type (WT) and the cryptochrome 1 (Cry1) hy4 mutant of A. thaliana Col-0 plants grown under blue light (BL) were studied. HIL and UV-B treatments led to decreases in the photosynthetic rate (Pn), photochemical activity of PSII (FV/FM) and PSII performance index (PIABS) of WT and mutant plants grown under high-intensity BL (HBL) and moderate intensity BL (MBL). However, in HBL plants, the decrease in the photosynthetic activity in hy4 plants was significantly greater than that in WT plants. In addition, hy4 HBL plants demonstrated lowered UAP and carotenoid contents as well as lower activity of APX and GPX enzymes. The difference in the decline in the photosynthetic activity of WT and hy4 plants grown at MBL in response to HIL was nonsignificant, while that in response to UV-B was small. We assume that the deficiency in cryptochrome 1 under HIL irradiation disrupts the interaction between HY5 and HFR1 transcription factors and photoreceptors, which affects the transcription of light-induced genes, such as CAB1, PSY and PAL1 linked to carotenoid and flavonoid biosynthesis. It was concluded that PA stress resistance in WT and hy4 plants depends on the light intensity and reduced stress resistance of hy4 at HBL, is likely linked to low UAP and carotenoid contents as well as lowered APX and GPX enzyme activities in hy4 mutants. [Display omitted] •The stress resistance of the photosynthetic apparatus in wild-type Arabidopsis was higher than that in the hy4 mutant.•The contents of leaf pigments and antioxidant enzyme activities were higher in the wild type than in the mutant.•Damage to the photosynthetic apparatus depended on the blue light intensity and cryptochrome 1 content.