In vivo evaluation of oxidative stress and biochemical alteration as biomarkers in glass clover snail, Monacha cartusiana exposed to zinc oxide nanoparticles

• Published in: Environmental pollution (1987) Vol. 257; p. 113120
• Main Authors: Abdel-Halim, Khaled Y., Osman, Safaa R., Abdou, Gehan Y.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2020
• Subjects: Animals; Biomarkers; Biomarkers - metabolism; Catalase - metabolism; Enzyme Activation - drug effects; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Glutathione Transferase - metabolism; Lipid Peroxidation - drug effects; Metal Nanoparticles - toxicity; Monacha cartusiana; Oxidative stress; Oxidative Stress - drug effects; Snails; Snails - drug effects; Snails - enzymology; Zinc Oxide - toxicity; Zinc oxide nanoparticles; Biomarkers; Oxidative stress; Monacha cartusiana; Snails; Zinc oxide nanoparticles
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2019.113120

Oxidative stress is considered a main commonly reported mechanism of nanoparticles toxicity, so this study aimed to evaluate oxidative stress and biochemical alterations in the haemolymph and digestive gland of snail, Monacha cartusiana exposed to sublethal concentrations of zinc oxide nanoparticles (ZnONPs) for 14 days (d). The results indicated that, ZnONPs induced significant increases in lipid peroxidation (LPO) and lactate dehydrogenase (LDH) in treated animals and did not return to normal levels after recover period. A significant decline of glutathione peroxidase (GPx), glutathione-S-transferase (GST) activities, and glutathione (GSH) content in the haemolymph and digestive gland of snails was observed when compared with control. A significant increase was observed in catalase (CAT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities of treated animals. In general, nano-materials are able to induce oxidative stress in exposed animals. The present findings indicate that, alterations of antioxidant enzyme activities, increase of LPO, LDH, and reducing of GSH content and GST, GPx activities are recognized to oxidative stress and cell damage. This species could be considered a good bioindicator to assess nano-materials exposure. [Display omitted] •Oxidative stress is considered a main commonly reported mechanism of nanoparticles toxicity.•ZnONPs induced adverse effects on land snail; Monnacha cartusiana.•Land snails could be considered good bioindicators to assess nano-materials exposure.