Determination of uric acid in human urine by ion chromatography with conductivity detector

• Published in: Chinese chemical letters Vol. 22; no. 3; pp. 342 - 345
• Main Authors: Zhao, Fu Yong, Wang, Zong Hua, Wang, Hui, Zhao, Rui, Ding, Ming Yu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.03.2011; Metrohm China Ltd. Beijing, Beijing 100005, China; Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China; Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, China%Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, China%Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China
• Subjects: Ion chromatography; Uric acid; Urine; 样品预处理; 测定分析; 电导检测器; 相对标准偏差; 离子色谱法; 阳离子交换柱; Urine; Ion chromatography; Uric acid
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2010.10.008

A simple,fast,precise and eco-friendly analytical method for the determination of uric acid（UA） in human urine by ion chromatography（IC） was established.The sample pretreatment was not required,only needed centrifugation and filtration.The separation was carried out on a cation exchange column with 2.0 mmol/L nitric acid as mobile phase at the flow-rate 1.0 mL/min.A non-suppressed conductivity detector was used.The IC analysis time for one run was within 10 min under the optimized IC condition.The detection limits were 0.5μg/L（S/N = 3） for uric acid.The recovery was 100.1%while the relative standard deviation （RSD） was 1.8%from 10 measurements.