An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1

• Published in: Biomedicine & pharmacotherapy Vol. 96; pp. 545 - 550
• Main Authors: Salehi, Shima, Mansoori, Behzad, Mohammadi, Ali, Davoudian, Sadaf, Musavi Shenas, Seyed Mohammad Hossein, Shajari, Neda, Majidi, Jafar, Baradaran, Behzad
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.12.2017
• Subjects: E-cadherin; Epithelial to mesenchymal transition; MMPs; Snail-1; Urinary bladder cancer; Vimentin; Epithelial to mesenchymal transition; Vimentin; E-cadherin; Urinary bladder cancer; MMPs; Snail-1
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2017.10.044

Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer. Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression. The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate. These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.