Direct Comparison between Forster Resonance Energy Transfer and Light-Induced Triplet-Triplet Electron Resonance Spectroscopy

To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Fo'rster resonanc...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Chemical Society Vol. 145; no. 42; pp. 22859 - 22865
Main Authors Bertran, Arnau, Morbiato, Laura, Sawyer, Jack, Dalla Torre, Chiara, Heyes, Derren J., Hay, Sam, Timmel, Christiane R., Di Valentin, Marilena, De Zotti, Marta, Bowen, Alice M.
Format Journal Article
LanguageEnglish
Published WASHINGTON Amer Chemical Soc 25.10.2023
American Chemical Society
Subjects
Chemistry
Chemistry, Multidisciplinary
Communication
Physical Sciences
Science & Technology
SINGLE-MOLECULE FRET
DISTANCE MEASUREMENTS
DEER
ECHO
DIPOLAR EPR SPECTROSCOPY
MODEL
METAL-IONS
BINDING
SPIN LABELS
MODULATION
Online AccessGet full text

Cover

More Information
Summary:To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Fo'rster resonance energy transfer (FRET) on exactly the same molecules with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet-triplet electron resonance method with 2-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.3c04685