Overexpression of a cotton (Gossypium hirsutum) WRKY gene, GhWRKY34, in Arabidopsis enhances salt-tolerance of the transgenic plants

• Published in: Plant physiology and biochemistry Vol. 96; pp. 311 - 320
• Main Authors: Zhou, Li, Wang, Na-Na, Gong, Si-Ying, Lu, Rui, Li, Yang, Li, Xue-Bao
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.11.2015
• Subjects: Adaptation, Physiological - genetics; Amino Acid Sequence; Arabidopsis - genetics; Arabidopsis - physiology; Cotton (Gossypium hirsutum); Genes, Plant; Gossypium - genetics; High salinity; Molecular Sequence Data; Overexpression; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - physiology; Salinity; Salt tolerance; Sequence Homology, Amino Acid; Stress, Physiological; Subcellular Fractions; Up-Regulation; WRKY protein; Cotton (Gossypium hirsutum); High salinity; Salt tolerance; Overexpression; WRKY protein
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2015.08.016

Soil salinity is one of the most serious threats in world agriculture, and often influences cotton growth and development, resulting in a significant loss in cotton crop yield. WRKY transcription factors are involved in plant response to high salinity stress, but little is known about the role of WRKY transcription factors in cotton so far. In this study, a member (GhWRKY34) of cotton WRKY family was functionally characterized. This protein containing a WRKY domain and a zinc-finger motif belongs to group III of cotton WRKY family. Subcellular localization assay indicated that GhWRKY34 is localized to the cell nucleus. Overexpression of GhWRKY34 in Arabidopsis enhanced the transgenic plant tolerance to salt stress. Several parameters (such as seed germination, green cotyledons, root length and chlorophyll content) in the GhWRKY34 transgenic lines were significantly higher than those in wild type under NaCl treatment. On the contrary, the GhWRKY34 transgenic plants exhibited a substantially lower ratio of Na+/K+ in leaves and roots dealing with salt stress, compared with wild type. Growth status of the GhWRKY34 transgenic plants was much better than that of wild type under salt stress. Expressions of the stress-related genes were remarkably up-regulated in the transgenic plants under salt stress, compared with those in wild type. Based on the data presented in this study, we hypothesize that GhWRKY34 as a positive transcription regulator may function in plant response to high salinity stress through maintaining the Na+/K+ homeostasis as well as activating the salt stress-related genes in cells. •GhWRKY34 is localized to the cell nucleus.•Overexpression of GhWRKY34 in Arabidopsis enhances plant salt-tolerance.•GhWRKY34 transgenic plants exhibited a substantially lower ratio of Na+/K+ than wild type.•The stress-related genes were up-regulated in the GhWRKY34 transgenic Arabidopsis under salt stress.