Peculiarities of studying an isolated neuron by the method of laser interference microscopy

Actual aspects of using a new method of laser interference microscopy (LIM) for studying nerve cells are discussed. The peculiarities of the LIM display of neurons are demonstrated by the example of isolated neurons of a pond snail Lymnaea stagnalis. A comparative analysis of the images of the cell...

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Bibliographic Details
Published inQuantum electronics (Woodbury, N.Y.) Vol. 36; no. 9; pp. 874 - 878
Main Authors Yusipovich, Alexander I, Novikov, Sergey M, Kazakova, Tatiana A, Erokhova, Liudmila A, Brazhe, Nadezda A, Lazarev, Grigory L, Maksimov, Georgy V
Format Journal Article
LanguageEnglish
Published United States IOP Publishing 30.09.2006
Subjects
60 APPLIED LIFE SCIENCES
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGY
CELL CONSTITUENTS
CYTOLOGY
CYTOPLASM
ELECTROMAGNETIC RADIATION
INTERFERENCE
INVERTEBRATES
LASER RADIATION
MICROSCOPY
MOLLUSCS
NERVE CELLS
OPTICAL MICROSCOPY
PHYSIOLOGY
RADIATIONS
SNAILS
SOMATIC CELLS
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Summary:Actual aspects of using a new method of laser interference microscopy (LIM) for studying nerve cells are discussed. The peculiarities of the LIM display of neurons are demonstrated by the example of isolated neurons of a pond snail Lymnaea stagnalis. A comparative analysis of the images of the cell and subcellular structures of a neuron obtained by the methods of interference microscopy, optical transmission microscopy, and confocal microscopy is performed. Various aspects of the application of LIM for studying the lateral dimensions and internal structure of the cytoplasm and organelles of a neuron in cytology and cell physiology are discussed. (laser biology)
ISSN:1063-7818
1468-4799
DOI:10.1070/QE2006v036n09ABEH013408