Heterologous complementation studies reveal the solute transport profiles of a two-member nucleobase cation symporter 1 (NCS1) family in Physcomitrella patens

• Published in: Plant physiology and biochemistry Vol. 100; pp. 12 - 17
• Main Authors: Minton, Janet A., Rapp, Micah, Stoffer, Amanda J., Schultes, Neil P., Mourad, George S.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.03.2016
• Subjects: Bryopsida - genetics; Bryopsida - metabolism; Genetic Complementation Test; Nucleobase cation symporter 1; Nucleobase Transport Proteins - genetics; Nucleobase Transport Proteins - metabolism; Physcomitrella patens; Plant Proteins - genetics; Plant Proteins - metabolism; Purine and pyrimidine; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Transport; Purine and pyrimidine; Transport; Physcomitrella patens; Nucleobase cation symporter 1
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2015.12.014

As part of an evolution-function analysis, two nucleobase cation symporter 1 (NCS1) from the moss Physcomitrella patens (PpNCS1A and PpNCS1B) are examined – the first such analysis of nucleobase transporters from early land plants. The solute specificity profiles for the moss NCS1 were determined through heterologous expression, growth and radiolabeled uptake experiments in NCS1-deficient Saccharomyces cerevisiae. Both PpNCS1A and 1B, share the same profiles as high affinity transporters of adenine and transport uracil, guanine, 8-azaguanine, 8-azaadenine, cytosine, 5-fluorocytosine, hypoxanthine, and xanthine. Despite sharing the same solute specificity profile, PpNCS1A and PpNCS1B move nucleobase compounds with different efficiencies. The broad nucleobase transport profile of PpNCS1A and 1B differs from the recently-characterized Viridiplantae NCS1 in breadth, revealing a flexibility in solute interactions with NCS1 across plant evolution. •P. patens genome contains two nucleobase cation symporter 1 loci, PpNCS1A and 1B.•Heterologous complementation in yeast reveals broad transport profile.•PpNCS1A and 1B move adenine, uracil, guanine, cytosine, hypoxanthine and xanthine.