An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase,...

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Published inGreen chemistry : an international journal and green chemistry resource : GC Vol. 20; no. 1; pp. 230 - 242
Main Authors Zhang, Liaoyuan, Singh, Raushan, D, Sivakumar, Guo, Zewang, Li, Jiahuan, Chen, Fanbing, He, Yuanzhi, Guan, Xiong, Kang, Yun Chan, Lee, Jung-Kul
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 2018
Subjects
Acetoin
Alcohols
Biofuels
Butanediol
Butanol
Catalysis
Dehydration
Dehydrogenase
Dehydrogenases
Ethanol
Green chemistry
NADH
NADH oxidase
Nicotinamide adenine dinucleotide
Upgrading
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Summary:Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.
ISSN:1463-9262
1463-9270
DOI:10.1039/C7GC02898A