Evaluation of Anthocyanin Production in White and Purple Maize (Zea mays L.) Using Methyl Jasmonate, Phosphorus Deficiency and High Concentration of Sucrose
Anthocyanins are plants metabolites that are recognized by its red/purple coloration produced in flowers, seeds and leaves. These molecules are potentially important to the industry for its antioxidant capacity, disease prevention and as a natural dye. Currently, the production of anthocyanins is ca...
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Published in | Cereal research communications Vol. 47; no. 4; pp. 604 - 614 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.12.2019
Akademiai Kiado |
Subjects | |
Online Access | Get full text |
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Summary: | Anthocyanins are plants metabolites that are recognized by its red/purple coloration produced in flowers, seeds and leaves. These molecules are potentially important to the industry for its antioxidant capacity, disease prevention and as a natural dye. Currently, the production of anthocyanins is carried out using
in vitro
culture of
Vitis vinifera
and its yield is increased by using elicitors or stress factors.
Zea mays
is relevant due to its high content of cyanidin-3-β-glucoside anthocyanin. In the present study the production of cyanidin-3-β-glucoside was evaluated with different mechanisms of elicitation using
in vivo
and
in vitro
culture of purple and white maize varieties. The highest callus induction (85%) for white maize was obtained in MS medium supplemented with 2 mg/L of 2,4-Dichlorophenoxyacetic acid, while for purple maize (93%) was obtained in N6 medium with 2 mg/L of 2,4-Dichlorophenoxyacetic acid, using germinated seed as explant for both varieties. Methyl jasmonate was evaluated as an elicitation tool, however no cyanidin-3-β-glucoside was found to be accumulated or produced
in vitro
. In contrast, using germinated seeds and radicle tissue, elicitation using phosphorus deficiency treatment produced the highest cyanidin-3-β-glucoside accumulation (0.06 mg g–1) in white maize. No elicitation and further production of anthocyanins was found when purple maize were used using this method. Therefore,
in vivo
elicitation in white maize is a potential method to produce a stable anthocyanin that could be optimized for future applications. |
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ISSN: | 0133-3720 1788-9170 |
DOI: | 10.1556/0806.47.2019.35 |