Unveiling the biodiversity of lipid species in Corynebacteria- characterization of the uncommon lipid families in C. glutamicum and pathogen C. striatum by mass spectrometry

• Published in: Biochimie Vol. 178; pp. 158 - 169
• Main Authors: Wang, Hay-Yan J., Tatituri, Raju V.V., Goldner, Nicholas K., Dantas, Gautam, Hsu, Fong-Fu
• Format: Journal Article
• Language: English
• Published: France Elsevier B.V 01.11.2020
• Subjects: CDP-Diacylglycerol; Cord Factors - chemistry; Corynebacterium - chemistry; Corynebacterium glutamicum - chemistry; Diglycerides - chemistry; Humans; Lipid Metabolism; Lipidomics; Lipids - chemistry; Lipids - classification; Lipids - isolation & purification; Microbial lipids; Multiple stage linear ion-trap mass spectrometry; Mycolic acid-containing PG; Phosphatidylglycerols - chemistry; Spectrometry, Mass, Electrospray Ionization; CDP-Diacylglycerol; Lipidomics; Multiple stage linear ion-trap mass spectrometry; Microbial lipids; Mycolic acid-containing PG
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2020.07.002

Uncommon lipids in biotechnologically important Corynebacterium glutamicum and pathogen Corynebacterium striatum in genus Corynebacterium are isolated and identified by linear ion-trap multiple stage mass spectrometry (LIT MSn) with high resolution mass measurement. We redefined several lipid structures that were previously mis-assigned or not defined, including cytidine diphosphate diacylglycerol (CDP-DAG), glucuronosyl diacylglycerol (GlcA-DAG), (α-d-mannopyranosyl)-(1 → 4)-(α-D-glucuronyl diacyglycerol (Man-GlcA-DAG), 1-mycolyl-2-acyl-phosphatidylglycerol (MA-PG), acyl trehalose monomycolate (acyl-TMM). We also report the structures of mycolic acid, phosphatidylglycerol, phosphatidylinositol, cardiolipin, trehalose dimycolate lipids in which many isomeric structures are present. The LIT MSn approaches afford identification of the functional group, the fatty acid substituents and their regiospecificity in the molecules, revealing the biodiversities of the lipid species in two Corynebacterium strains that have played very different and important roles in human nutrition and health. •LIT MSn with high resolution mass spectrometric approaches toward characterization of several classes of uncommon lipids in Corynebacteria are described. .•The lipid structures in Corynebacteria previously mis-assigned are redefined.•LIT MSn analysis revealed the richness and complexity of the lipid species in Corynebacterium genus.•Differences in the lipid profiles between C. glutamicum and pathogen C. striatum were found.