Molecular identification and antifungal susceptibility testing of Candida species isolated from dental plaques

• Published in: Journal de mycologie médicale Vol. 28; no. 3; pp. 433 - 436
• Main Authors: Aslani, N., Abastabar, M., Hedayati, M.T., Shokohi, T., Aghili, S.R., Diba, K., Hosseini, T., Bahrami, B., Ebrahimpour, A., Salehi, M., Taheri Sarvtin, M., Haghani, I., Vafaei Moghaddam, M.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.09.2018
• Subjects: Antifungal Agents - therapeutic use; Antifungal susceptibility; Candida - classification; Candida - drug effects; Candida - genetics; Candida - isolation & purification; Candida spp; Candidiasis, Oral - drug therapy; Candidiasis, Oral - microbiology; Dental Plaque - microbiology; Dental plaques; Drug Resistance, Fungal - genetics; Humans; Iran; Microbial Sensitivity Tests; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Iran; Candida spp; Antifungal susceptibility; Dental plaques
• ISSN: 1156-5233; 1773-0449
• DOI: 10.1016/j.mycmed.2018.05.006

The aim of the present study was to provide insight into the prevalence and susceptibility profiles of Candida species isolated from the dental plaque of Iranian immunocompetent patients. As a biofilm, Candida species are responsible for several disorders common to the oral cavity including gingivitis, dental caries, periodontitis, and the less common severe systemic infections specifically in immunosuppressed individuals. PCR-RFLP was performed to identify yeasts isolated from the dental plaques of 40 immunocompetent patients. Moreover, antifungal susceptibility testing was performed in according to CLSI guidelines (M27-A3). Among 40 yeasts isolated from the dental plaques of immunocompetent patients, Candida albicans was the most common species (92.5%), followed by P. kudriavzevii (7.5%). It is the first isolation of P. kudriavzevii from dental plaques and the first evaluation of antifungal effect of the new imidazole, luliconazole and echinocandins against these samples worldwide. Luliconazole, voriconazole, amphotericin B and anidulafungin showed the best activity with the lowest geometric mean (GM) 0.03, 0.06, 0.08 and 0.09μg/ml, respectively, followed by miconazole (0.14μg/mL), caspofungin (0.24μg/mL) fluconazole (0.38μg/mL) and itraconazole (0.5μg/mL). The current study demonstrated luliconazole and echinocandins displayed excellent activity against all Candida isolates from dental plaques, presenting promising and potent alternative for all oral Candidiasis.