Preparation of novel chiral stationary phase based on click chemistry for ligand exchange chromatography

• Published in: Chinese chemical letters Vol. 20; no. 11; pp. 1345 - 1347
• Main Authors: Fu, Chun Mei, Shi, Hong Yu, Qian, Guang Sheng, Li, Zhang Wan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2009; Graduate School of the Chinese Academy of Sciences,Beijing 100049,China; West China School of Pharmacy,Sichuan University,Chengdu 610041,China%Chengdu Institute of Organic Chemistry,Chinese Academy of Sciences,Chengdu 610041,China
• Subjects: Chiral stationary phase; Click chemistry; CSP封装; dl-amino acids; Ligand exchange chromatography; 化学应用; 对映选择性; 手性固定相; 色谱固定相; 配体交换色谱; dl-amino acids; Chiral stationary phase; Click chemistry; Ligand exchange chromatography; DE-amino acids
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2009.05.011

Click chemistry was applied to immobilize L-proline derivative onto azide-modified silica gel to give a novel chiral stationary phase （denoted as click-CSP） for ligand exchange chromatography. The developed protocol combines the benefits of operational simplicity, exceptionally mild conditions and high surface loadings. The enantioselectivity α of some DL-arnino acids on the click- CSP were found to be in the range from 1.13 to 3.46. The chromatographic resolutions of some DL-amino acids and the stability study firmly illustrate the potential of click chemistry for preparation chiral stationary phase for ligand exchange chromatography.