Distribution of Gastrin in Human Digestive Organs Demonstrated by Direct Immunofluorescence

1. Antiserum was prepared in the rabbit against synthetic human gastrin I conjugated with bovine serum albumin. The IgG fraction of this specific antiserum was labelled with FITC (fluoresceine isothiocyanate). With a direct immunofluorescence technique the distribution of gastrin in the human digest...

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Bibliographic Details
Published inArchivum histologicum japonicum Vol. 37; no. 1; pp. 59 - 72
Main Authors TOBE, Takayoshi, HENMI, Kimio, FUKUCHI, Kozo, CHEN, Shin-Tse, TACHIBANA, Shinro
Format Journal Article
LanguageEnglish
Published Japan International Society of Histology and Cytology 1974
Subjects
Animals
Colon - analysis
Digestive System - analysis
Duodenum - analysis
Fluorescent Antibody Technique
Freezing
Gastric Mucosa - analysis
Gastrins - analysis
Gastrins - immunology
Glycine - pharmacology
Humans
Ileum - analysis
Immune Sera
Immunodiffusion
Jejunum - analysis
Pancreas - analysis
Parotid Gland - analysis
Rabbits - immunology
Rectum - analysis
Serum Albumin, Bovine
Submandibular Gland - analysis
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Summary:1. Antiserum was prepared in the rabbit against synthetic human gastrin I conjugated with bovine serum albumin. The IgG fraction of this specific antiserum was labelled with FITC (fluoresceine isothiocyanate). With a direct immunofluorescence technique the distribution of gastrin in the human digestive tract and associated glands was investigated with special reference to extragastric gastrin. 2. Gastrin-containing cells were numerous in the antral mucosa of the human stomach especially in the prepyloric region (2-4cm proximal to the pylorus). In the body and cardia of the stomach no gastrin-containing cells were seen. 3. Some gastrin-containing cells were seen in the duodenum but fewer than in the antrum and they decreased distally. 4. No gastrin-containing cells were seen in the esophagus, jejunum, ileum and colon. 5. In the pancreas numerous autofluorescent granules were seen, but no specific gastrin-containing cells. Nor could these cells be found in the parotid and submandibular glands. 6. The release of gastrin after immersion in 0.5M glycine solution was investigated. A slight decrease of the specifically fluorescent substance could be seen. A complete release of the substance followed freezing in an isopentane bath in aqueous nitrogen.
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ISSN:0004-0681
DOI:10.1679/aohc1950.37.59