Effects of TGF-β1 and IL-1β on expression of ADAMTS enzymes and TIMP-3 in human intervertebral disc degeneration

• Published in: Experimental and therapeutic medicine Vol. 6; no. 6; pp. 1522 - 1526
• Main Authors: WANG, SHI-LONG, YU, YONG-LIN, TANG, CHAO-LIANG, LV, FEI-ZHOU
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.12.2013; Spandidos Publications UK Ltd
• Subjects: a disintegrin and metalloproteinase with thrombospondin motifs; Cell growth; interleukin-1β; Kinases; nucleus pulposus; Studies; tissue inhibitor of metalloproteinase 3; transforming growth factor-β1; United States > US; a disintegrin and metalloproteinase with thrombospondin motifs; transforming growth factor-β1; tissue inhibitor of metalloproteinase 3; nucleus pulposus; interleukin-1β
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2013.1348

The aim of this study was to investigate the effects of transforming growth factor-β1 (TGF-β1) and interleukin-1β (IL-1β) on the expression of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) enzymes and their inhibitor, tissue inhibitor of metalloproteinase 3 (TIMP-3), in human intervertebral disc (IVD) degeneration. Cells from patients with IVD degeneration were cultured with Dulbecco's modified Eagle's medium with Ham's F12 nutrient mixture (DMEM/F12) medium at 37°C in a 5% CO2 incubator. Cell proliferation was measured by cell counting kit-8 assays with varying concentrations of TGF-β1 and IL-1β in a time-response experiment. The mRNA and protein expression levels of ADAMTS-4, ADAMTS-5 and TIMP-3 were detected with qPCR and western blot analysis, respectively. The present study demonstrated that TGF-β1 promoted nucleus pulposus (NP) cell proliferation, decreased the expression of ADAMTS-4 and -5 and increased the expression of TIMP-3. By contrast, the IL-1β treatment inhibited NP cell proliferation and significantly increased the expression of ADAMTS-4 and -5. However, IL-1β appeared to have no marked effect on the expression of TIMP-3. This study suggests that TGF-β1 and IL-1β are involved in the synthesis and degradation of the extracellular matrix and may act as potential therapeutic targets for the prevention or reversal of IVD degeneration.